Purolite™ DurA™ Cycle A50

Reduces cost of goods, improving purification and extending lifetime.

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Meeting Modern Demands of Cleaning-in-Place Protocols

As downstream purification advances, the use of enhanced cleaning-in-place (CIP) protocols has expanded to maintain product integrity and ensure high-quality biopharmaceuticals.

Purolite DurA Cycle A50 is engineered to purify monoclonal antibodies (mAbs) and associated protein constructs under variable cleaning strategies.

Compared to standard affinity resins, Purolite DurA Cycle A50 offers mild pH elution, addressing aggregation and subsequent purity challenges.

Designed to reduce susceptibility to protein A leakage, Purolite DurA Cycle A50 offers an extended resin lifetime, lowering the cost per gram.

Features

High Capacity 50 µm bead size

Alkaline stable up to 0.5 M NaOH

mAb pH elution up to 4.5

High dynamic binding capacity up to 80 g/L

Manufactured using Jetting technology

Typical Physical & Chemical Properties

Matrix

Highly cross-linked agarose

Dynamic binding capacity

Up to 80 mg hIgG/ml

Average Particle Size

50 µm

Pressure/flow specifications

Up to 200 cm/h (30 x 20 cm)

Particle Size Range

95% between 35 - 90 µm

pH Stability (Working Range)

3 - 12

pH Stability, CIP (Short Term)

2 - 13

Recommended storage

2 - 8 °C in 20% ethanol

Product Data

Dynamic Binding Capacity

Purolite DurA Cycle A50 exhibits high dynamic binding capacities for conventional monoclonal antibodies, up to 80 g/l at a 10-minute residence time. Patented Jetting technology, coupled with the latest Protein A ligand technology, allows for high capacity even at low residence times. The resin is uniquely suited to address the demands of modern bioprocessing, from high-titer cell cultures to continuous chromatography formats.

Alkaline Stability

0.1 M Sodium hydroxide (NaOH) exhibits high efficiency in removing bound proteins,nucleic acids, and lipids from bioprocess resins, alleviating the risk of fouling on heavily burdened Protein A columns. Higher concentrations of sodium hydroxide may be used between campaigns and when more substantial cleaning is required.

Purolite DurA Cycle A50 is alkaline stable for over 60 hours of exposure to 0.5 M NaOH, and over 300 hours of exposure to 0.1 M NaOH.

Milder pH Elution Window

The ligand design allows for elution at a wider range of pH when compared to conventional Protein A chromatography. Allowing for a wider operational window to address aggregation concerns and increase purity clearance within the capture step.