Praesto® Jetted A50 HipH
A high capacity, alkaline-stable protein A resin designed for elution of Fc-containing molecules at higher pH levels.
The unique properties of Praesto Jetted A50 HipH address challenges in eluting pH-sensitive mAbs and Fc-containing proteins, which can become unstable at pH levels typically used for elution with other Protein A resins (3 – 3.5).
This resin provides alkaline stability, capacity of 60 g/L for polyclonal human IgG and elution up to pH 5. This creates a wider operational window for your Protein A step, facilitating rapid process development and processing of all Fc-containing molecules by:
Up to 60 mg/ml dynamic binding capacity
> 95% recovery and purity
Elution up to pH 5
Aligns with proven polishing steps
Uniform beads and high mass transfer
Innovative High pH Elution Technology
The unique ligand design allows for elution at a wider range of pH (3 - 5) when compared to conventional protein A chromatography without impacting yield, purity or elution volumes.
Sodium hydroxide (NaOH) exhibits high efficiency in removing bound proteins, nucleic acids, and lipids from bioprocess resins, alleviating the risk of fouling on heavily burdened Protein A columns. Praesto Jetted A50 HipH maintains excellent capacity after exposure to 0.1 M NaOH for 120 hours, facilitating efficient CIP and longer resin lifetime.
Highly cross linked agarose
Average particle size (d50ᵥ), μm
Particle size range (micron)
95% between 35-90 µm
Dynamic binding capacity
Up to ~60 mg hIgG/ml resin
Up to 200 cm/h (30 x 20 cm column)
pH stability, CIP (short term)
3 - 14
pH stability, working range
3 - 12
2 – 8°C in 20% ethanol
Praesto Jetted A50 HipH is manufactured using our patented Jetting technology, which produces consistent agarose beads with a uniform particle size distribution. A secure, fast, green technology, Jetting means supply chain security, reduced lead times, and faster mAb processing, all whilst contributing towards your sustainability goals.