How to Care for Strong Acid Cation Exchange Resins - Hydrogen Cycle
Resins are usually delivered in the sodium form.
- Exhaust the resin by passing 2 BV of 4% NaOH through a column of resin at a rate of 2 BV/h
- Rinse with 5 BV of DI water at a rate of 2 BV/h
- Regenerate the resin by passing 4 BV of HCl or H2SO4 through a column of resin at a flow rate of 2 BV/h
- 5 BV 5% HCl = 5.5 eq/lBV
- 5% H2SO4 = 4.1 eq/l
4. Rinse the resin with 5 BV of DI water at a flow rate of 2 BV/h
5. Repeat the above procedure at least once.
BV refers to the volume of resin used.
For example, if using one liter of resin, then 1 BV (or 1 bed volume) equals one liter.
How to Care for Strong Acid Cation Resins - Sodium Cycle
- Exhaust the resin with raw water by passing 6 BV of 2% CaCl2 solution at a flow rate of 2 BV/h = 2.2 eq/l
- Rinse with 5 BV of DI water at a rate of 2 BV/h
- Regenerate the resin by passing 2 BV of 10% NaCl at a rate of 2 BV/h
- Rinse with 5 BV of DI water at a rate of 2 BV/h
- Repeat the above process at least once
How to Care for Weak Acid Cation Exchange Resins
Resins are usually delivered in the hydrogen form.
- Exhaust the resin by passing 5 BV of 4% NaOH hrough a column of the resin at a rate of 2 BV/h (a dosage equivalent to 5 eq/L). There is potentially high volume change for weak acid cation (WAC) resins in changing from hydrogen to sodium form.
Examples:
Purolite C104Plus 60%, Purolite C115E 100%
- Rinse with 5 BV of DI water at a rate of 2 BV/h
- Regenerate the resin by passing 5 BV of 5% HCl or 5% or H2SO4 at a rate of 2 BV/h
- Rinse with 5 BV of DI water at a rate of 2 BV/h
- Repeat above procedure at least once
Note: 1 BV = 1 liter of water running through 1 liter of resin or 7.48 US gallons through 1 ft³ of resin.
How to Care for Strong Base Anion Exchange Resins
Resins are usually delivered in the salt (exhausted) form.
- Regenerate the resin by passing 3 BV of 4% NaOH through a column of resin
at a rate of 2 BV/h
- Rinse with 5 BV DI water at a rate of 2 BV/h
- Exhaust with 2 BV of 4% HCI or H2SO4 solution (Note: acid solution can be replaced with NaCI solution)
- Rinse again
How to Care for Weak Base Anion Exchange Resins
Resins are usually delivered in the free-base form.
- Exhaust the resin by passing 2 BV of 5% HCl or 5% H2SO4 at a rate of 2 BV/h through a column of the resin
- Rinse with 5 BV DI water at a rate of 2 BV/h
- Regenerate the resin by passing 3 BV of 5% NaOH or 5% NH4OH at a rate of 2 BV/h
- Rinse with 5 BV of DI water at a rate of 2 BV/h
- Repeat the above process at least twice
How to Care for NSF/ANSI 44 & NSF/ANSI 61 Certified Resins
Please refer to the WQA website for pre-use directions.
How to Care for PraestoTM SP200 Agarose Resin
Before use, Praesto SP200 should be prepared by the following procedure.
- Equilibrate out of shipping buffer into packing buffer - typically 400 mM NaCl.
- Pack the column according to Purolite Praesto packing instructions.
- Once packed, rinse with 2-5 bed volumes of 400 mM NaCl.
- Rinse with 2-5 bed volumes of water or running buffer as required for the purification process.
Cleaning should be carried out regularly, as a wash with 2-5 bed volumes of 0.5 M NaOH followed by neutralization with 0.1 M acetate and a 2-5 bed volume re-equilibration into water or running buffer.
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It is strongly recommended that the product water is tested and confirmed to be fully in specification before it is used for direct human consumption or for the intended food treatment.
Note: Depending on storage time and conditions, additional pre-treatment may be required.
