Praesto 70 CH1

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CH1 Affinity Resin

Recent years have seen the expansion of antibody variants used in the development of therapeutic treatments, including the use of bispecifics. However, traditional methods of purification have proven inefficient.

Part of the Purolite™ Affinity Resin Toolbox

Purolite’s Praesto 70 CH1 is a 70μm (micron) agarose-based affinity resin designed to purify specialized mAbs such as bispecifics and recombinant antibody fragments, and can be used when Fc or light-chain binding is not viable.

The Purolite Difference

Developed in partnership with Repligen, an industry leader and partner in life sciences, Praesto 70 CH1 provides high capacity and alkaline stability.
Manufactured using Purolite’s patented Jetting technology, Praesto 70 CH1 offers uniform beads to ensure quality and performance.


Up to 2X capacity compared to established CH1 affinity resins

Highest clean-in-place (CIP) alkaline stability of any CH1 binding resin

Supports process efficiencies to reduce long-term cost of goods

Product Data

NaOH Stability

Relative binding capacity of Praesto 70 CH1 after 0.1 M NaOH exposure.

Praesto 70 CH1 offers improved caustic stability compared to established CH1 resins, allowing the adoption of standard 0.1M NaOH CIP protocols.
Dynamic Binding Capacity
Capacity data for an IgG1 subclass monoclonal antibody on Praesto 70 CH1 at 3, 4.8, 6, and 10-minute residence time.

Praesto 70 CH1 offers higher binding capacity than any established CH1 resin, delivering favorable process economics for bispecifics and fusion proteins.


Praesto 70 CH1 Datafile


Laboratory Scale Column Packing Instructions


Pilot Scale Column Packing Instructions


Praesto 70 CH1 IFU


Praesto 70 CH1

Typical Physical & Chemical Properties


Highly cross-linked agarose

Dynamic binding capacity

40 g/L mAb (IgG1) Fab* at 6 minute residence time


70 µm

Pressure/flow specifications

400 cm/h at 1.8 bar (30 x 20 cm)


> 95% between 43-122 µm

pH Stability (Working Range)

2 - 13

pH Stability, CIP (Short Term)

2 - 13


2 - 8 °C in 20% ethanol