Purolite Praesto SP Range

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Overview

The strong chemical and physical stability of Purolite Praesto Jetted Q resins provide a long functional life and in turn, long-term reductions in operating costs.

Q resins maintain charge and subsequent capacity, allowing for binding and elution across a wide pH range. High resolution and selectivity allows for demanding separations while maintaining high yields.

The high and robust dynamic binding capacity of Q resins enables improvements to productivity and scalability of ion exchange processes.

Features

Up to 90 mg/ml dynamic binding capacity

Up to 550 cm/h flow velocity

Available in various particle sizes (35, 45, 65, and 90 μm)

Uniform beads and high mass transfer

Product Data

Dynamic Binding Capacity
Praesto base matrices have porosities optimized for medium-sized proteins, resulting in robust binding capacities. High capacity is especially important to facilitate processing of high concentration eluates from protein A resins.
 
Pressure Flow
The uniformity of jetted beads, allows flexibility in selecting high surface area, high capacity, and high-resolution products without diminishing flow properties. 
 

Resources

Purolite Praesto SP/Q Data File

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Column Packing Instructions

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SP/Q Product Brochure

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Purolite Praesto™ Jetted Q35 Purolite Praesto™ Q45 Purolite Praesto™ Q65 Purolite Praesto™ Q90

Typical Physical & Chemical Properties

マトリックス

Cross-linked agarose

官能基

CH2N+C+(CH3)3

イオン交換容量 - 中

0.11 - 0.18 mmol Cl⁻/ml

平均粒径 (d50ᵥ), μm

35 µm

Flow velocity

Up to 120 cm/h (30 x 20 cm)

結合容量 mg/mL-R(接触時間6分)

> 80 mg/ml BSA

運転時pH安定性 (短期)

2 - 14

運転時pH安定性 (長期)

2 - 12

運転温度

4 - 30 °C

化学的安定性

All commonly used aqueous buffers, 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol

避ける

Oxidizing agents, cationic detergents

保管

20% ethanol, 4 - 30°C

マトリックス

Cross-linked agarose

官能基

CH2N+C+(CH3)3

イオン交換容量 - 中

0.09 - 0.18 mmol Cl⁻/ml

平均粒径 (d50ᵥ), μm

45 µm

Flow velocity

Up to 200 cm/h (20 x 20 cm)

結合容量 mg/mL-R(接触時間6分)

≥ 70 mg BSA/ml

運転時pH安定性 (短期)

2 - 14

運転時pH安定性 (長期)

2 - 12

運転温度

4 - 30 °C

化学的安定性

All commonly used aqueous buffers. 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol

避ける

Oxidising agents, anionic detergents

推奨保管条件

20% ethanol at 4 – 30 °C

マトリックス

Cross-linked agarose

官能基

CH2N+C+(CH3)3

イオン交換容量 - 中

0.09 - 0.18 mmol Cl⁻/ml

平均粒径 (d50ᵥ), μm

65 µm

Flow velocity

Up to 350 cm/h (20 x 20 cm) cm/h

結合容量 mg/mL-R(接触時間6分)

≥ 60 mg BSA/ml

運転時pH安定性 (短期)

2 - 14

運転時pH安定性 (長期)

2 - 12

運転温度

4 - 30 °C

化学的安定性

All commonly used aqueous buffers. 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol

避ける

Oxidising agents, anionic detergents

推奨保管条件

20% ethanol at 4 – 30 °C

マトリックス

Cross-linked agarose

官能基

CH2N+C+(CH3)3

イオン交換容量 - 中

0.09 - 0.18 mmol Cl⁻/ml

平均粒径 (d50ᵥ), μm

90 µm

Flow velocity

Up to 550 cm/h (20 x 20 cm)

結合容量 mg/mL-R(接触時間6分)

≥50 mg BSA/ml

運転時pH安定性 (短期)

2 - 14

運転時pH安定性 (長期)

2 - 12

運転温度

4 - 30 °C

化学的安定性

All commonly used aqueous buffers. 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol

避ける

Oxidising agents, anionic detergents

推奨保管条件

20% ethanol at 4 – 30 °C