Essentially, a column that is poorly packed can lead to expensive process disruptions and ultimately, loss of a valuable product. 

In these short instructions, methods for packing both Praesto® AP and Praesto® Jetted A50 are described. Methods for packing laboratory scale up to small process scale (20 cm diameter) columns are detailed below in the two documents.

Praesto Chromatography Resin Column Packing

However, at the process development stage on laboratory scale, the design of the column used and packing approach can significantly impact the results of screening evaluations. A resin cannot be truly evaluated if not packed efficiently.

In the short investigation below, we have evaluated two of the most common formats used to pack columns at small scale; GE Healthcare Tricorn™ and Kinesis Omnifit® columns.