A variety of base matrix materials can be used for mAb purification. Among these, agarose is widely considered the best material available.  It is useful as it does not absorb biomolecules or their contaminants to any significant extent, has excellent flow properties, and can tolerate extreme levels of pH and ionic strength.

Agarose is a polysaccharide, generally extracted from certain types of red seaweed –Gracilaria and Gelidium. Agarose is one of the two principal components of agar and is purified by removing its other component, agaropectin. It is frequently used in molecular biology for the separation of large molecules, especially DNA, by electrophoresis. Agarose is an inherently soft material, so we use sophisticated emulsification and crosslinking technologies to increase rigidity. This ensures excellent pressure flow performance.
 

Agarose has a variety of characteristics that makes it ideal for antibody/protein purification, such as:

• It is extremely hydrophilic, resulting in minimal unspecific binding
   
• High porosity (92-96%) enables higher capacity resins
   
• It is very stable under alkaline conditions, enabling cleaning with sodium hydroxide