Purolite™ Praesto™ SP
Agarose-based cation exchange chromatography resins for purification of monoclonal antibodies, recombinant proteins, and other biomolecules.
Matrix
Cross-linked agarose
Groupe fonctionnel
CH2CH2CH2SO3-
Ionic capacity - medium
0.11 - 0.18 mmol H⁺/ml
Average particle size (d50ᵥ), μm
35 µm
Flow velocity
Up to 120 cm/h (30 x 20 cm)
Binding Capacity mg/mL Resin at 6’ Residence Time
> 90 mg/ml lgG
Operating pH Stability (Short Term)
3 - 14
Operating pH Stability (Long-Term)
4 - 12
Working Temperature
4 - 30 °C
Chemical stability
All commonly used aqueous buffers, 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Avoid
Oxidizing agents, cationic detergents
Storage
20% ethanol, 0.2M sodium acetate, 4 - 30 °C
Matrix
Cross-linked agarose
Groupe fonctionnel
CH2CH2CH2SO3 -
Ionic capacity - medium
0.11 - 0.18 mmol H⁺/ml
Average particle size (d50ᵥ), μm
45 µm
Flow velocity
Up to 200 cm/h (20 x 20 cm)
Binding Capacity mg/mL Resin at 6’ Residence Time
≥ 80 mg lgG/ml
Operating pH Stability (Short Term)
3 - 14
Operating pH Stability (Long-Term)
4 - 12
Working Temperature
4 - 30 °C
Chemical stability
All commonly used aqueous buffers. 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Avoid
Oxidising agents, cationic detergents
Recommended storage
20% ethanol, 0.2 M sodium acetate, 4 – 30 °C
Matrix
Cross-linked agarose
Groupe fonctionnel
CH2CH2CH2SO3 -
Ionic capacity - medium
0.11 - 0.18 mmol H⁺/ml
Average particle size (d50ᵥ), μm
65 µm
Flow velocity
Up to 350 cm/h (20 x 20 cm)
Binding Capacity mg/mL Resin at 6’ Residence Time
≥ 70 mg lgG/ml
Operating pH Stability (Short Term)
3 - 14
Operating pH Stability (Long-Term)
4 - 12
Working Temperature
4 - 30 °C
Chemical stability
All commonly used aqueous buffers. 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Avoid
Oxidising agents, cationic detergents
Recommended storage
20% ethanol, 0.2 M sodium acetate, 4 – 30 °C
Matrix
Cross-linked agarose
Groupe fonctionnel
CH2CH2CH2SO3 -
Ionic capacity - medium
0.11 - 0.18 mmol H⁺/ml
Average particle size (d50ᵥ), μm
90 µm
Flow velocity
Up to 550 cm/h (20 x 20 cm)
Binding Capacity mg/mL Resin at 6’ Residence Time
≥ 50 mg lgG/ml
Operating pH Stability (Short Term)
3 - 14
Operating pH Stability (Long-Term)
4 - 12
Working Temperature
4 - 30 °C
Chemical stability
All commonly used aqueous buffers. 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Avoid
Oxidising agents, cationic detergents
Recommended storage
20% ethanol, 0.2 M sodium acetate, 4 – 30 °C