Bioprocessing
Accelerate your biologic drug development with Praesto®
chromatography resins.

Accelerate your biologic drug development with Praesto®
chromatography resins.
In qualità di leader mondiale nella tecnologia delle resine, sviluppiamo e produciamo piccole perline che vengono utilizzate nelle industrie più regolamentate del mondo per separare, rimuovere o recuperare elementi e composti molto specifici.
Per saperne di piùCon 40 anni di esperienza nella produzione e 30 anni di esperienza normativa, forniamo tecnologie di separazione, purificazione ed estrazione all'avanguardia per supportare le applicazioni di cromatografia e biocatalisi nel settore sanitario e delle scienze della vita.
*(Attualmente solo in inglese)
Siamo il leader mondiale nella tecnologia di separazione, purificazione ed estrazione a base di resina, che fornisce soluzioni sostenibili per il nostro ambiente, le aziende e l'assistenza sanitaria.
Scopri di piùThe only agarose-based resins with uniform particle sizes. For unrivalled dependability and consistency in downstream process performance, scale-up and column packing characteristics.
I nostri team di assistenza e supporto tecnico sempre pronti fanno il passo in più per essere la tua risorsa più affidabile. Siamo qui per voi.
Monoclonal antibody (mAb) therapeutics are widely-used to treat ailments including cancer, rheumatoid arthritis, dementia and diabetes. Agarose-based chromatography resins are a vital technology for biopharmaceutical developers to obtain optimal purity in downstream processing of monoclonal antibodies and recombinant proteins.
The purification of commercially-available monoclonal antibodies (mAbs) on the market today is typically performed in three chromatography steps. Purolite offer industry-leading solutions for each step.
Protein A Affinity Chromatography
A Protein A affinity resin is utilized as an initial antibody capture step. Praesto Protein A resins deliver exceptionally high purity (>99%) and yield in a single, efficient step.
Cation Exchange Chromatography
A Sulphopropyl- (SP) functionalized cation exchange resin in 'bind and elute' mode is used to remove aggregates and HCP (Host Cell Proteins).
Anion Exchange Chromatography
A Quarternary Amine (Q) functionalized anion exchange resin is used in 'flow-through' mode to remove trace contaminates and ensure sufficient viral clearance.
A variety of base matrix materials can be used for mAb purification. Among these, agarose is widely considered the best material available. It is useful as it does not absorb biomolecules or their contaminants to any significant extent, has excellent flow properties, and can tolerate extreme levels of pH and ionic strength.
Agarose is a polysaccharide, generally extracted from certain types of red seaweed –Gracilaria and Gelidium. Agarose is one of the two principal components of agar and is purified by removing its other component, agaropectin. It is frequently used in molecular biology for the separation of large molecules, especially DNA, by electrophoresis. Agarose is an inherently soft material, so we use sophisticated emulsification and crosslinking technologies to increase rigidity. This ensures excellent pressure flow performance.
Agarose has a variety of characteristics that makes it ideal for antibody/protein purification, such as:
All Praesto products are manufactured with our patented Jetting technology. For faster, greener and more consistent bioprocessing.
Purolite's goal is to secure supply, enable lot-to-lot consistency and minimize the impact of change. As such, we have made continual strategic investments to offer industry-leading security of supply and business continuity plans.
Anion Exchange Chromatography - Agarose Base
Cation Exchange Chromatography - Agarose Base