Purolite™ Praesto™ Q

Agarose-based anion exchange chromatography resins for purification of monoclonal antibodies, recombinant proteins, and other biomolecules.

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Overview

The strong chemical and physical stability of Purolite Jetted Q resins provide a long functional life and in turn, long-term reductions in operating costs.

Q resins maintain charge and subsequent capacity, allowing for binding and elution across a wide pH range. High resolution and selectivity allows for demanding separations while maintaining high yields.

The high and robust dynamic binding capacity of Q resins enables improvements to productivity and scalability of ion exchange processes.

Features

Up to 90 mg/ml dynamic binding capacity

Up to 550 cm/h flow velocity

Available in various particle sizes (35, 45, 65, and 90 μm)

Uniform beads and high mass transfer

Typical Physical & Chemical Properties

Matrix

Cross-linked agarose

Grupo funcional

CH₂N+C+(CH₃)₃

Capacidad iónica - medina

0.11 - 0.18 mmol Cl⁻/ml

Average particle size (d₅₀ᵥ), μm

35 µm

Flow velocity

Up to 120 cm/h (30 x 20 cm)

Binding Capacity mg/mL Resin at 6’ Residence Time

> 80 mg/ml BSA

Operating pH Stability (Short Term)

2 - 14

Operating pH Stability (Long-Term)

2 - 12

Working Temperature

4 - 30 °C

Estabilidad química

All commonly used aqueous buffers, 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol

Evitar

Oxidizing agents, cationic detergents

Storage

20% ethanol, 4 - 30°C

Matrix

Cross-linked agarose

Grupo funcional

CH₂N+C+(CH₃)₃

Capacidad iónica - medina

0.09 - 0.18 mmol Cl⁻/ml

Average particle size (d₅₀ᵥ), μm

45 µm

Flow velocity

Up to 200 cm/h (20 x 20 cm)

Binding Capacity mg/mL Resin at 6’ Residence Time

≥ 70 mg Ml de BSA

Operating pH Stability (Short Term)

2 - 14

Operating pH Stability (Long-Term)

2 - 12

Working Temperature

4 - 30 °C

Estabilidad química

All commonly used aqueous buffers. 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol

Evitar

Oxidising agents, anionic detergents

Almacenaje recomendado

20% ethanol at 4 – 30 °C

Matrix

Cross-linked agarose

Grupo funcional

CH₂N+C+(CH₃)₃

Capacidad iónica - medina

0.09 - 0.18 mmol Cl⁻/ml

Average particle size (d₅₀ᵥ), μm

65 µm

Flow velocity

Up to 350 cm/h (20 x 20 cm) cm/h

Binding Capacity mg/mL Resin at 6’ Residence Time

≥ 60 mg Ml de BSA

Operating pH Stability (Short Term)

2 - 14

Operating pH Stability (Long-Term)

2 - 12

Working Temperature

4 - 30 °C

Estabilidad química

All commonly used aqueous buffers. 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol

Evitar

Oxidising agents, anionic detergents

Almacenaje recomendado

20% ethanol at 4 – 30 °C

Matrix

Cross-linked agarose

Grupo funcional

CH₂N+C+(CH₃)₃

Capacidad iónica - medina

0.09 - 0.18 mmol Cl⁻/ml

Average particle size (d₅₀ᵥ), μm

90 µm

Flow velocity

Up to 550 cm/h (20 x 20 cm)

Binding Capacity mg/mL Resin at 6’ Residence Time

≥50 mg Ml de BSA

Operating pH Stability (Short Term)

2 - 14

Operating pH Stability (Long-Term)

2 - 12

Working Temperature

4 - 30 °C

Estabilidad química

All commonly used aqueous buffers. 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol

Evitar

Oxidising agents, anionic detergents

Almacenaje recomendado

20% ethanol at 4 – 30 °C

Product Data

Dynamic Binding Capacity

Purolite base matrices have porosities optimized for medium-sized proteins, resulting in robust binding capacities. High capacity is especially important to facilitate processing of high concentration eluates from protein A resins.

Pressure Flow

The uniformity of jetted beads, allows flexibility in selecting high surface area, high capacity, and high-resolution products without diminishing flow properties.

Purolite™ Praesto™ Q