APPLICATION NOTE

Contrasting Dynamic Binding Capacity Across Affinity Resins

Protein A affinity resins are most commonly used to perform the capture step in therapeutic drug purification workflows. However, this step contributes significantly to the overall production cost of protein purification. 

To reduce production costs, high-capacity protein A resins are preferred as they bind more efficiently to the target molecule. Unsurprisingly, dynamic binding capacity (DBC) is a critical consideration when adopting a protein A resin for monoclonal antibody (mAb) purification.

This study compares the DBC of two Purolite™ protein A resins and two competitor products for the purification of three classes of antibody-related molecules.