Purolite Praesto™ Jetted A50 HipH | Protein Purification at a higher pH

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Bioprocessing

Accelerate your biologic
drug development with
Purolite™ chromatography resins.

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Saúde e Ciências da Vida

Com 40 anos de experiência em fabricação e 30 anos de experiência regulatória, fornecemos tecnologias líderes de separação, purificação e extração para apoiar aplicações de cromatografia e biocatálise em saúde e ciências da vida.
(Atualmente apenas em inglês)

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Reducing Aggregation for Purification of pH-Sensitive Molecules

Complex molecules, including fc-based IgGs, offer unique benefits to therapeutic drug development. However, these acid-sensitive molecules become unstable at typical elution levels (pH 3–3.5), significantly decreasing product yield.

Purolite Jetted A50 HipH is engineered to support the elution of Fc-containing molecules at higher pH levels, enhancing monomer recovery.

By addressing protein aggregation in the screening process, Purolite Jetted A50 HipH reduces the burden on downstream polishing steps to remove product impurities.

Features

High dynamic binding capacity up to 60 g/L

Alkaline stable up to 0.1 M NaOH

mAb pH elution up to 5

High capacity 50 μm bead

Purolite Praesto™ Jetted A50 HipH

Typical Physical & Chemical Properties

Estrutura Polimérica

Highly cross linked agarose

Grupo funcional

Protein A

Average particle size (d₅₀ᵥ), μm

50 µm

Particle size range (micron)

95% between 35-90 µm

Dynamic binding capacity

Up to ~60 mg hIgG/ml resin

Pressure/flow specifications

Up to 200 cm/h (30 x 20 cm column)

pH para estabilidade, CIP (curto prazo)

2 - 13

Estabilidade do pH, faixa de trabalho

3 - 12

Armazenamento Recomendado

2 - 8°C in 20% ethanol / 2% benzyl alcohol. Shipped in 20% ethanol / 2 % benzyl alcohol on request.

Product Data

Innovative High pH Elution Technology

The unique ligand design allows for elution at a wider range of pH (3 - 5) when compared to conventional protein A chromatography without impacting yield, purity or elution volumes.

Alkaline Stability

Sodium hydroxide (NaOH) exhibits high efficiency in removing bound proteins, nucleic acids, and lipids from bioprocess resins, alleviating the risk of fouling on heavily burdened Protein A columns. Purolite Jetted A50 HipH maintains excellent capacity after exposure to 0.1 M NaOH for 120 hours, facilitating efficient CIP and longer resin lifetime.

Dynamic Binding Capacity

The uniform jetted bead maintains high mass transfer and high capacity even at low residence times. Purolite Jetted A50 HipH shows comparable performance for polyclonal IgG capacity to Purolite Jetted A50.

Resources

Purolite Praesto Jetted A50 HipH Datafile

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Purolite Praesto Jetted A50 HipH IFU

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Column Packing Instructions

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Purolite™ Praesto™ Jetted A50 HipH