Praesto® Jetted A50
A highly cross-linked Praesto agarose base matrix with the NGL-Impact™ A ligand.
Praesto Jetted A50 is a high-performance agarose-based protein A resin that delivers exceptional performance in terms of capacity, pressure flow and lifetime.
Designed to meet the mAb processing demands of tomorrow, today, the combination of a highly cross-linked Praesto agarose base matrix with the NGL-Impact™ A ligand from Repligen® provides increased capacity and excellent alkaline stability, reducing your bioburden risk and improving your process efficiencies from the same manufacturing footprint.
Up to 70 mg/ml mAb and up to 80 mg/ml hIgG
Alkaline Stable. At over 100 hours exposure (NaOH) to 0.1 M NaOH
The only manufacturer of uniform particle size agarose resins
Excellent Pressure Flow Properties
The uniform particle size and highly cross-linked agarose base bead provides increased surface area without severely impacting pressure flow properties. Praesto Jetted A50 has excellent flow properties, with up to 200 cm/h achievable in a standard mAb platform process. This has been verified in process scale columns.
Sodium hydroxide exhibits high efficiency in removing bound proteins, nucleic acids, and lipids from bioprocess resins, alleviating the risk of fouling on heavily burdened Protein A columns. Praesto Jetted A50 is alkaline stable for over 100 hours of exposure to 0.1 M NaOH, and over 30 hours of exposure to 0.5 M NaOH.
Dynamic Binding Capacity
Praesto Jetted A50 exhibits high dynamic binding capacities for conventional monoclonal antibodies, up to 70 g/L at a 10-minute residence time. Patented Jetting technology, coupled with the latest Protein A technology, allows for high capacity at even low residence times. The resin is uniquely suited to address the demands of modern bioprocessing, from high-titre cell cultures to continuous chromatography formats.
Highly cross linked agarose
Spherical, uniform size beads supplied in 20% ethanol slurry. On request 2% benzylalcohol
Dynamic binding capacity
Up to 70 mg mAb/ml resin
Up to 80 mg hIgG/ml resin
(10 minute residence time)
Average Particle Size
Particle size range (micron)
>95% between 35-90 µm
Up to 200 cm/h (30 x 20 cm)
pH stability, working range
3 - 12
pH stability, CIP (short term)
2 - 13
2- 8 °C in 20% ethanol, 2% benzyl alcohol. Shipped in 20% ethanol, 2 % benzyl alcohol on request.
Praesto Jetted A50 HipH is manufactured using our patented Jetting technology, which produces consistent agarose beads with a uniform particle size distribution. A secure, fast, green technology, Jetting means supply chain security, reduced lead times, and faster mAb processing, all whilst contributing towards your sustainability goals.