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Praesto®
Jetted A50

Highly Cross Linked Agarose Protein A Affinity Resin

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Praesto Jetted A50 is an agarose-based novel Protein A affinity resin with industry-leading performance characteristics, and utilizes a new ligand from Repligen Corporation - NGL-ImpactTM A.

Praesto Jetted A50 beads are manufactured using patented Jetting technology, an innovative process that produces uniform size agarose beads with a very narrow particle size distribution. These uniform particle sizes deliver superior performance characteristics over traditional resins. 

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Principal Applications

  • Protein purification (20-500 kDa)
  • MAb Purification

Advantages

  • Uniform particle size
  • Repligen® NGL-ImpactTM A ligand
  • Highest dynamic binding capacity (~80g/l)
  • Exceptional alkaline stability
  • Enhanced pressure/flow performance
  • Manufactured with reduced environmental impact

Regulatory Approvals

  • Manufactured under GMP conditions

Typical Packaging

  • Bulk Resin
  • Production-Scale OPUS® Columns
  • OPUS® Robocolumns®
  • OPUS® MiniChrom Columns
  • HT Columns

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical, uniform size beads supplied in 20% ethanol slurry. On request 2% benzylalcohol
Functional Group Protein A
Average Particle Size (d50ᵥ), μm 50 µm
Particle size range (micron) 95% between 35-90 µm (Uniformity coefficient = <1.3)
Dynamic Binding Capacity (min.)- 3 minutes residence time ~ 50 g/l at 3 mins residence time and up to ~80 g/l at 10 mins residence time
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) > 300 cm/h
pH stability, CIP (short term) 2 - 14
pH stability, working range 3 - 12
Recommended Storage 2 - 8 °C
Recommended Storage 20% ethanol