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Praesto®
Jetted A50

Highly Cross Linked Agarose Protein A Affinity Resin

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Praesto Jetted A50 is an agarose-based novel Protein A affinity resin with industry-leading performance characteristics, and utilizes a new ligand from Repligen Corporation - NGL-ImpactTM A. Praesto Jetted A50 beads are manufactured using patented 'Jetting' technology, an innovative process that produces uniform size agarose beads with a very narrow particle size distribution.

These uniform particle sizes deliver superior performance characteristics over traditional resins. 

Principal Applications

  • Protein purification (20-500 kDa)
  • MAb Purification

Advantages

  • Uniform particle size
  • Repligen® NGL-ImpactTM A ligand
  • Highest dynamic binding capacity (~80g/l)
  • Exceptional alkaline stability
  • Enhanced pressure/flow performance
  • Manufactured with reduced environmental impact

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • Bulk Resin
  • Production-Scale OPUS® Columns
  • OPUS® Robocolumns®
  • OPUS® MiniChrom Columns
  • HT Columns

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical, uniform size beads supplied in 20% ethanol slurry. On request 2% benzylalcohol
Functional Group Protein A
Average Particle Size (d50ᵥ), μm 50 µm
Particle size range (micron) 95% between 35-90 µm (Uniformity coefficient = <1.3)
Dynamic Binding Capacity (min.)- 3 minutes residence time ~ 50 g/l at 3 mins residence time and up to ~80 g/l at 10 mins residence time
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) > 300 cm/h
pH stability, CIP (short term) 2 - 14
pH stability, working range 3 - 12
Recommended Storage 2 - 8 °C
Recommended Storage 20% ethanol

The statements, technical information and recommendations contained herein are believed to be accurate as of the date hereof. Since the conditions and methods of use of the product and of the information referred to herein are beyond our control, Purolite expressly disclaims any and all liability as to any results obtained or arising from any use of the product or reliance on such information; NO WARRANTY OF FITNESS FOR ANY PARTICULAR PURPOSE, WARRANTY OF MERCHANTABILITY OR ANY OTHER WARRANTY, EXPRESSED OR IMPLIED, IS MADE CONCERNING THE GOODS DESCRIBED OR THE INFORMATION PROVIDED HEREIN. The information provided herein relates only to the specific product designated and may not be applicable when such product is used in combination with other materials or in any process. Nothing contained herein constitutes a license to practice under any patent and it should not be construed as an inducement to infringe any patent and the user is advised to take appropriate steps to be sure that any proposed use of the product will not result in patent infringement.