In this Question-and-Answer session, we sit down with Penny Hamlyn, Senior Business Development Manager and Course Lead, as she shares insight into Purolite, an Ecolab Company’s Chromatography Masterclass; an exclusive five-part course that covers the fundamentals of chromatography and protein purification techniques from the lab bench to commercial scale.  

Penny, who has always worked in the field of purification, has twenty-five years of experience in chromatography. Previously working as an AKTA specialist with Cytiva for over twenty years, before working with the Astex Pharmaceuticals purification team and the Roche scale-up team.

What is the Purolite Chromatography Masterclass?

The chromatography masterclass is a series of five seminars designed to engage and educate chromatography. Each seminar goes through various techniques, applications, system configurations, and media chemistry which helps learners to select the perfect chromatography media for their specific needs, with a spotlight on standard protein purification methods. 


What is the layout of this course?

There are five pre-recorded seminars around forty-five minutes long, which can be watched at your leisure, with each session accompanied by a range of supporting slides, studies, and whitepapers to help continue your learning.

The series starts with the Fundamentals of Chromatography, which provides an excellent introduction for those new to the field. The seminar begins with expression and extraction and explains chromatography principles, key terms and concepts, and what to consider when designing a purification. The following four seminars concentrate on one chromatography technique, building on the basics already learned to give in-depth knowledge to design and optimize your purification.


Can you share some highlights or key topics viewers can expect to see in the series?

One of the key highlights of the masterclass is the way the information is shared, starting with the basics and building knowledge as the series progresses, ensuring that the learner is never out of their depth and the course is both enjoyable and useful. We have also included the skills you need to ensure your purification is fit for purpose. What do I mean by this? Well, you have to consider very different things if your purification has the potential to scale up for manufacture.


Who should attend the Chromatography Masterclass?

The masterclass is ideal for a range of skill levels: those who are new to chromatography, those in need of a refresher, and those who have good chromatography skills but limited knowledge of what to consider when scaling up for manufacture. Attendees can expect to gain invaluable knowledge that will empower them to tackle the challenges of protein purification with confidence.


How can I register?

Once you have registered, you can watch all of the seminars OnDemand with access to hours of course material, supporting documentation, and direct access to our team of Field Applications Scientists, who are happy to answer any technical questions you may have.

View Purolite’s online training portal here.