Purolite DurA Cycle A50

Driving innovation and performance while reducing cost of goods.

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Addressing Modern mAb Purification Challenges

The latest protein A resin from Ecolab, Purolite Resins, Purolite DurA Cycle A50 aims to maximize productivity and improve process economics.

Purolite DurA Cycle A50 improves purification performance with high capacities, which can help to enable a lower cost per gram of commercial drug product. Building on the innovation of Ecolab, Purolite Resins Praesto Jetted A50 HipH, Purolite DurA Cycle A50 provides a wider elution pH window to address aggregate clearance.

The Purolite Difference

Developed in partnership with Repligen, an industry leader and partner in life sciences, Purolite DurA Cycle A50 provides high capacity and alkaline stability. Manufactured using Purolite’s patented Jetting technology, Purolite DurA Cycle A50 offers uniform beads to ensure quality and performance.

Features

High Capacity 50 µm bead size

Alkaline Stable

Mild pH elution window

High dynamic binding capacity of up to 80 g/L

Product Data

Dynamic Binding Capacity

Purolite DurA Cycle A50 exhibits high dynamic binding capacities for conventional monoclonal antibodies, up to 80 g/l at a 10-minute residence time. Patented Jetting technology, coupled with the latest Protein A ligand technology, allows for high capacity even at low residence times. The resin is uniquely suited to address the demands of modern bioprocessing, from high-titer cell cultures to continuous chromatography formats.

Alkaline Stability

0.1 M Sodium hydroxide (NaOH) exhibits high efficiency in removing bound proteins,nucleic acids, and lipids from bioprocess resins, alleviating the risk of fouling on heavily burdened Protein A columns. Higher concentrations of sodium hydroxide may be used between campaigns and when more substantial cleaning is required.

Purolite DurA Cycle A50 is alkaline stable for over 60 hours of exposure to 0.5 M NaOH, and over 300 hours of exposure to 0.1 M NaOH.

Milder pH Elution Window

The ligand design allows for elution at a wider range of pH when compared to conventional Protein A chromatography. Allowing for a wider operational window to address aggregation concerns and increase purity clearance within the capture step.

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