New Product

DurA Cycle A50
Driving innovation and performance while
reducing cost of goods.

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mAb & protein purification
mAb & protein purification
Overview Products
Products
Affinity Chromatography Resins
Affinity Chromatography Resins
Overview Purolite DurA Cycle A50 Purolite Praesto™ 70 CH1 Purolite Praesto™ AP+80 Purolite Praesto™ Jetted A50 HipH Purolite Praesto™ Jetted A50
IEX Chromatography Resins
IEX Chromatography Resins
Overview Purolite Praesto™ Jetted SP Purolite Praesto™ Jetted Q
Jetting Technology Technical Support
Tools & Services
Tools & Services
Knowledge Hub Chromatography Column Packing Calculator Throughput and Productivity Calculator Field Application Services

Purification Technologies

As a global leader in resin technology, we develop and manufacture small beads that are used in the most regulated industries in the world to separate, remove or recover very specific elements and compounds.

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Product Types
Product Types
Adsorbents
Adsorbents
Overview Adsorbents for Enzyme Immobilization Adsorbents for Gases and Organic Vapors Adsorbents of Organic Pollutants Citrus Juice Debittering Corn Sweetener Refining Inorganic Salt Separation Using Adsorbents Patulin Removal Polyphenol Recovery
Catalyst Chelation Resins Chromatographic Separation Resins Inert Spacer Polymers Mixed Bed Resins
Mixed Bed Resins
Overview Mixed Bed Nuclear Mixed Bed Ultrapure Water
Shallow Shell™ Resins Strong Acid Cation Resin Strong Base Anion Resin Weak Acid Cation Resin
Weak Acid Cation Resin
Overview Weak Acid Cation Macroporous Weak Acid Cation Porous
Weak Base Anion Resin
Weak Base Anion Resin
Overview Weak Base Anion Gel Weak Base Anion Macroporous
Industries
Industries
Carbon Capture Chemical Processing and Petrochemical Food and Beverage
Food and Beverage
Overview Dairy Flavors Food Ingredients Fruit Juice
Fruit Juice
Overview Citrus Juice Debittering Patulin Removal Polyphenolic Compound Recovery
Gelatin and Collagen Organic Acid Purification Sugar
Sugar
Overview Beet Sugar Refining Cane Sugar Refining
Sweetener
Sweetener
Overview Corn Sweetener Refining
Additional Food and Beverage
Hydrometallurgy Metals Plating Microelectronics Oil and Gas Potable and Groundwater
Potable and Groundwater
Overview Arsenic Nitrate PFAS TOC, Bromide and DBP Perchlorate
Power
Power
Overview Fossil Nuclear Green Hydrogen
Pulp and Paper
Applications
Applications
Adsorption Catalysis Condensate Polishing Demineralization Metal Removal and Recovery Organics Removal Softening Water Purity Solutions

Pharma and Medical

With 40 years of manufacturing expertise and 30 years of regulatory experience, we supply leading separation, purification and extraction technologies to support chromatography applications within the Pharma and Medical space.

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Pharmaceutical
Pharmaceutical
Overview APIs/Excipients Milling Media
Medical Devices and Diagnostics
Medical Devices and Diagnostics
Overview Biofluid Treatment Diagnostics
Nutraceuticals
Nutraceuticals
Overview Dairy Protein Isolation Supplement Ingredients Vitamin Purification

Purolite Resins

We are a world leader in resin-based separation, purification and extraction technology, that provides sustainable solutions for our environment, businesses and healthcare.

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About
About
About Us
About Us
Overview History
Locations Services Manufacturing Customer Support Research and Development Brands
Brands
CriticalResin™ Macronet™ Microlite™ Purofine™ Puromet™ Puromill™ Puropack™ PuroSorb™ Shallow Shell™ UltraClean™
Environmental Commitment
Resources
Resources
General Information Certifications
Certifications
Product Certifications Quality, Environment, Health and Safety
Terms of Sale Glossary and Definitions Case Studies
News & Events
News & Events
News Blog Webinars
Tools
Tools
Purolite Resin System Modeling (PRSM™) Unit Conversion Calculator
Replacing the art of column packing with science / Blog / purolite

The Art of Column Packing

Chromatographic column packing is often described as 'more art than science' when it comes to achieving consisent, optimal results. When packing is not performed correctly, you risk either expensive downtime from having to start your packing procedure over, or an overall drop in product quality from a non-homogeneous packed bed.

To have a robust, reliable and optimized chromatography process, you need to have a properly packed and qualified column, which ensures:
  • maximum efficiency;
  • high product yield;
  • high purity.
The key challenge though, is achieving consistent, optimal packing characteristics at your first attempt, whether you're packing columns at pilot or process scale.

 

Replacing the 'Art' with Science

We have expanded our Agarose resin manufacturing facility with the addition of a modern column packing suite, equipped with best-in-class pilot and process-scale equipment to match our best-in-class Praesto resin performance. 
 
The suite enables us to provide expert, hands-on training to increase your packing success rates at both pilot and process scales, as well as demonstrate scale-up performance of our full Praesto MAb purification range.

Whether you use manual or automated packing methods, talk to our Applications Support Team today for expert, hands-on guidance on:
  •  Packing
  •  Unpacking
  •  Column Priming
  •  Pressure Flow Testing
  •  Column Efficiency Testing
  •  Slurry and Buffer Preparation

Equipment

PILOT SCALE

ÄKTA pilot 600
Maximum flow of 600 ml/min (1200 ml/min in dual flow mode), capable of packing and running pressure flow studies in columns up to 10 cm ID.

Axichrom 70 Column
7 cm ID automated axial compression column for use with the ÄKTA pilot system.


PROCESS SCALE

ÄKTAprocess
A versatile platform offering maximum flow of 600 L/h, for use in packing and running pressure flow tests in columns up to 30 cm ID.

BPG300 Column
A 30 cm ID manual axial compression column for use with AKTA Process system.

Axichrom 600 Column
A 60 cm ID production scale column with automated axial compression, fully equipped with Axichrom master control system for column packing.

150 L slurry stirring vessel
Generates a homogenous slurry, ensuring a well-packed column and a robust and efficient purification process. For automated packing with Axichrom 600 column.

Quatroflow Pump
1200L/h capacity pump, enabling accurate pressure flow testing of the AxiChrom 600 column

The Praesto​​​​​ Range

Different chromatography resins each require different packing strategies for success. We provide guidance on packing:

Affinity Chromatography
Praesto Jetted A50
Praesto AP
Praesto APc
Praesto AC

Ion Exchange Chromatography
Praesto SP
Praesto Q
Praesto Jetted SP35
Praesto Jetted Q35