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Chromalite® MIDA is a chromatography resin used in protein purification by metal affinity. The resins show a high affinity for His-tag proteins after loading with metals such as iron, copper, cobalt, zinc and nickel.
Isolation of specific protein fractions from fermentation broth or protein mixtures by using cost-effective, scalable, sterilizable resins offers a significant increase in value and productivity. Thus, most of the recombinant proteins contain a histidine tag fused. Chromalite® MIDA was specifically designed to selectively capture His-tagged enzymes for both purification and biocatalytic processes.
This new range of IDA resins increases the potential use of a wide range of His-tagged enzymes in the manufacture of key compounds while minimizing the downstream processing cost by combining purification and immobilization in one simple step.
Chromalite MIDA Product Range
Resin volumes available:
A resin screening kit for affinity His-tag immobilization and enzyme purification. The SpectraChrom Kit includes six different metal-loaded resins that enable you to perform rapid screening during protein purification or metal affinity immobilization.
The kit includes 25ml of each Chromalite® MIDA resin:
Anion Exchange Chromatography - Polymer Base
Cation Exchange Chromatography - Polymer Base