Monoclonal Antibody Purification Kits

Monoclonal antibody (mAb) therapeutics are widely-used to treat ailments including cancer, rheumatoid arthritis, dementia and diabetes. Agarose-based chromatography resins are a vital technology for biopharmaceutical developers to obtain optimal purity in downstream mAb bioprocessing.

A Typical mAb Platform

The purification of commercially-available monoclonal antibodies (mAbs) on the market today is typically performed in three chromatography steps:

1. A Protein-A affinity resin is utilized as an initial antibody capture step. Praesto Protein A resins deliver exceptionally high purity (>99%) and yield in a single, efficient step.
2. A Sulphopropyl- (SP) functionalized cation exchange resin in 'bind and elute' mode is used to remove aggregates and HCP (Host Cell Proteins).
3. A Quarternary Amine (Q) functionalized anion exchange resin is used in 'flow-through' mode to remove trace contaminates and ensure sufficient viral clearance.

How do Protein and mAb Purification Resins Work?

Affinity chromatography is a method of separating proteins based on a highly specific, reversible interaction between an individual or group of proteins, and a suitable ligand (see selection table) attached to a chromatographic matrix.

Ion Exchange chromatography is used to separate molecules based on their net surface charge.

Anion exchange chromatography uses a positively charged ion exchange resin with an affinity for molecules having net negative surface charges.

Cation exchange chromatography uses a negatively charged ion exchange resin with an affinity for molecules having net positive surface charges.