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Protein A and Ion Exchange chromatography resins for optimum purity in downstream mAb bioprocessing.
Monoclonal antibody (mAb) therapeutics are widely-used to treat ailments including cancer, rheumatoid arthritis, dementia and diabetes. Agarose-based chromatography resins are a vital technology for biopharmaceutical developers to obtain optimal purity in downstream mAb bioprocessing.
A Typical mAb Platform
The purification of commercially-available monoclonal antibodies (mAbs) on the market today is typically performed in three chromatography steps:
How do Protein and mAb Purification Resins Work?
Affinity chromatography is a method of separating proteins based on a highly specific, reversible interaction between an individual or group of proteins, and a suitable ligand (see selection table) attached to a chromatographic matrix.
Ion Exchange chromatography is used to separate molecules based on their net surface charge.
Anion exchange chromatography uses a positively charged ion exchange resin with an affinity for molecules having net negative surface charges.
Cation exchange chromatography uses a negatively charged ion exchange resin with an affinity for molecules having net positive surface charges.
Evaluation Kit - MAb or Protein Purification