Affinity Chromatography HIS-TAG

Affinity chromatography is an efficient method for protein purification. Chromalite MIDA, a polymethacrylate based resins with iminodiacetic acid functionality, is an affinity-based resin for metal His-Tag protein purification.

Such resin is designed to have very high specificity for His-Tagged proteins, including enzymes, to allow for high purity and yield with a single step. The Chromalite MIDA can be loaded with different metals such as iron (Fe2+), zinc (Zn2+), cobalt (Co2+), copper (Cu2+) and nickel (Ni2+). In order to facilitate the screening, the Spectrachrom Kit offers all pre-loaded resins.