Reversed Phase Chromatography

Reversed-phase chromatography utilizes hydrophobic stationary phase, with a stronger affinity towards hydrophobic or non-polar compounds. Purolite reverse phase resins are mainly based on polystyrene-DVB based polymers with high porosity and surface area to create a highly hydrophobic surface for the interaction.

Reversed-phase chromatography uses a polar (aqueous) mobile phase where hydrophobic molecules will adsorb to the hydrophobic stationary phase, and hydrophilic molecules will pass through uninterrupted. Hydrophobic molecules are eluted from the resin by decreasing the polarity of the mobile phase via use of non-polar solvent such as alcohol, which reduces hydrophobic interactions.

The more hydrophobic the molecule, the higher the concentration of solvent needed to elute the molecule.

Reverse Phase Chromatography is a frequently used analytical method to quantify and separate various molecules such as betalactam antibiotics, flavors, polyphenols, vitamins, peptides, oligonucleotides and many more. Some applications where additional separations are needed such as ion exchange in a mixed mode type of separation (such as a reverse phase chromatography resin with ion exchange capabilities). Purolite offer such resin for the separations of peptides and oligonucleotides such as 10AD2S (cation exchanger) and 10AD2Q (anion exchanger).