Praesto® SP45 is an agarose based strong cation exchange resin designed for both large scale biomolecule purification and small scale applications. Praesto® SP45 can be used for purification of different biomolecules, including proteins, peptides and oligonucleotides. The particle size (45µm) makes Praesto® SP45 the smallest particle of the Praesto® family and thus particular useful for high resolution applications.


Principal Applications

  • Protein purification (20-500 kDa)
  • MAb Purification


  • Excellent dynamic binding capacities for high-productivity and scalability
  • Excellent pressure/flow performance
  • High resolution/selectivity
  • Excellent chemical and physical stability
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under GMP conditions

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Sulfopropyl
Chemical stability All commonly used aqueous buffers. 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Avoid Oxidising agents, cationic detergents
Ionic capacity - medium 0.11 - 0.16 mmol H⁺/ml
Ionic capacity - resin 0.11 - 0.16 mmol/ml
Particle Size - µm 45 µm
Dynamic Binding Capacity (min.) - 6 minutes residence time 80 lgG/ml
Dynamic Binding Capacity (min.)- 3 minutes residence time 80 hlgG/ml
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 200 cm/h
pH stability, CIP (short term) 3 - 14
pH stability, working range 4 - 13
Recommended Storage 4 - 30 °C
Recommended Storage 20% ethanol
Recommended Storage 0.2M sodium acetate