Praesto® Q45 is an agarose-based strong anion exchange resin. Praesto Q45 is designed for large-scale biomolecule purification, but is also an excellent choice for small scale applications. Praesto® Q45 can be used for purification of different biomolecules, including proteins, peptides and oligonucleotides. The particle size (45µm) makes Praesto® Q45 the smallest particle of the Praesto® family and thus particular useful for high resolution applications.
 

Principal Applications

  • Protein purification (20-500 kDa)
  • MAb Purification

Advantages

  • Excellent dynamic binding capacities for high-productivity and scalability
  • Excellent pressure/flow performance
  • High resolution/selectivity
  • Excellent chemical and physical stability
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under GMP conditions

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Quaternary amine
Chemical stability All commonly used aqueous buffers. 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Avoid Oxidising agents, anionic detergents
Ionic capacity - resin 0.14 - 0.18 mmol/ml
Ionic capacity - medium 0.14 - 0.18 mmol Cl⁻/ml
Particle Size - µm 45 µm
Dynamic Binding Capacity (min.) - 6 minutes residence time 70 BSA/ml
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 200 cm/h
pH stability, CIP (short term) 2 - 14
pH stability, working range 3 - 13
Recommended Storage 4 - 30 °C
Recommended Storage 20% ethanol