Praesto Pure is an agarose-based chromatography media designed for large-scale biomolecule purification. It is the ideal base matrix for the production of affinity chromatography resins, and is available in three separate particle sizes - 45 µm, 65 µm and 90 µm - making it the ideal choice for high-resolution, intermediate and capture steps. Praesto Pure can also be directly used for gel filtration of larger biomolecules such as viruses and plasmids.

Principal Applications

  • Protein purification (20-500 kDa)
  • Virus Application

Advantages

  • High productivity
  • High capacity
  • Enhanced pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • Bulk Resin
  • Production-Scale OPUS® Columns
  • OPUS® Robocolumns®
  • OPUS® MiniChrom Columns
  • HT Columns

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Not functionalized
Chemical stability All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents
Particle Size - µm 45, 65, 90 µm
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 200 cm/h
Exclusion limit for globular proteins 10 7 Daltons
pH stability, CIP (short term) 2 - 14
pH stability, working range 3 - 14
Recommended Storage 4 - 30 °C
Recommended Storage 20% ethanol