Highly cross linked pre-activated agarose base matrix
To support in the development and manufacture of biopharmaceuticals, Purolite has developed a range of pre-activated agarose resins. These resins enable manufacturers to couple their own ligands to develop affinity chromatography solutions. NHS pre-activated resins are available in three particle sizes - 45 μm, 65 μm and 90 μm.
Praesto NHS resins have been designed to offer a simple solution for the immobilization of ligands onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D proof of concept to larger scale bioprocess production columns. Praesto NHS resins couple ligands which have a primary amine. Praesto NHS incorporates a spacer chain which separates the ligand from the chromatography matrix enabling maximum accessibility to the ligand. The pre-activated NHS resin forms a stable amide linkage between the matrix and ligand resulting in very low ligand leakage and high caustic stability. A common application for NHS resins is immobilization of antibodies to create immunosorbents. Coupling is via primary amino groups. Many well documented references are publicly available.
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|Polymer Structure||Highly cross linked agarose|
|Chemical stability||After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents|
|Particle Size - µm||65 µm|
|Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar)||400 cm/h|
|Exclusion limit for globular proteins||10 7 Daltons|
|pH stability, CIP (short term)||2 - 13 (after coupling)|
|pH stability, working range||2 - 13 (after coupling)|
|Recommended Storage||100% IPA - 2 - 8 °C|
|Recommended Storage||20% EtoH (after coupling)|