Praesto NHS resins have been designed to offer a simple solution for ligand immobilization onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D to large-scale bioprocess production columns.

Praesto NHS resins couple ligands which have a primary amine. It incorporates a spacer chain which separates the ligand from the chromatography matrix enabling maximum accessibility to the ligand. The pre-activated NHS resin forms a stable amide linkage between the matrix and ligand resulting in very low ligand leakage and high caustic stability.

Praesto® Pre-Activated Chromatography Resins - FAQs

Principal Applications

  • Protein purification (20-500 kDa)
  • Production of customized affinity chromatography media

Advantages

  • High productivity
  • High capacity
  • Enhanced pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • Bulk Resin
  • Production-Scale OPUS® Columns
  • OPUS® Robocolumns®
  • OPUS® MiniChrom Columns
  • HT Columns

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group NHS-ester
Chemical stability After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents
Particle Size - µm 45, 65, 90 µm
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 200 cm/h
Exclusion limit for globular proteins 10 7 Daltons
pH stability, CIP (short term) 2 - 13 (after coupling)
pH stability, working range 2 - 13 (after coupling)
Recommended Storage 100% IPA - 2 - 8 °C
Recommended Storage 20% EtoH (after coupling)