Highly Cross Linked Pre-Activated Agarose Base Matrix
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Praesto NHS resins have been designed to offer a simple solution for ligand immobilization onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D to large-scale bioprocess production columns.
Praesto NHS resins couple ligands which have a primary amine. It incorporates a spacer chain which separates the ligand from the chromatography matrix enabling maximum accessibility to the ligand. The pre-activated NHS resin forms a stable amide linkage between the matrix and ligand resulting in very low ligand leakage and high caustic stability.
Praesto® Pre-Activated Chromatography Resins - FAQs
|Polymer Structure||Highly cross linked agarose|
|Chemical stability||After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents|
|Particle Size - µm||45, 65, 90 µm|
|Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar)||200 cm/h|
|Exclusion limit for globular proteins||10 7 Daltons|
|pH stability, CIP (short term)||2 - 13 (after coupling)|
|pH stability, working range||2 - 13 (after coupling)|
|Recommended Storage||100% IPA - 2 - 8 °C|
|Recommended Storage||20% EtoH (after coupling)|