Praesto NHS resins have been designed to offer a simple solution for ligand immobilization onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D to large-scale bioprocess production columns.

Praesto NHS resins couple ligands which have a primary amine. It incorporates a spacer chain which separates the ligand from the chromatography matrix enabling maximum accessibility to the ligand. The pre-activated NHS resin forms a stable amide linkage between the matrix and ligand resulting in very low ligand leakage and high caustic stability.

Praesto® Pre-Activated Chromatography Resins - FAQs

Principali applicazioni

  • Purificazione delle proteine
  • Production of customized affinity chromatography media

Vantaggi

  • High productivity
  • High capacity
  • Enhanced pressure/flow performance
  • Secure, validated supply and regulatory support

Certificazioni

  • Prodotto in condizioni cGMP

Imballi tipici

  • Bulk Resin
  • Production-Scale OPUS® Columns
  • OPUS® Robocolumns®
  • OPUS® MiniChrom Columns
  • HT Columns

Caratteristiche chimiche e fisiche tipiche:

Struttura del polimero Highly cross linked agarose
Aspetto Spherical beads
Gruppo funzionale NHS-ester
Stabilità chimica After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents
Distribuzione granulometrica - µm 45, 65, 90 µm
Pressione/flusso (min.) - a 3 bar in una colonna di 2.6 x 20 cm (impaccata a pressione a 4 bar) 200 cm/h
Limite di esclusione per proteine globulari 10 7 Daltons
Stabilità del pH, CIP (a breve termine) 2 - 13 (after coupling)
pH di stabilità, range operativo 2 - 13 (after coupling)
Conservazione/Stoccaggio consigliato 100% IPA - 2 - 8 °C
Conservazione/Stoccaggio consigliato 20% EtoH (after coupling)