Praesto® CNBr resins offer a simple solution for ligand immobilization onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D to large-scale bioprocess production columns.  Purolite Life Sciences can also offer custom solutions through our use of jetting technology, enabling the precise control of any bead size.

The use of Cyanogen bromide (CNBr) pre-activated base matrices is a well-established, rapid and familiar technique for the coupling of bio-specific ligands to generate affinity chromatography purification media. This choice of pre-activation chemistry is well suited for research, diagnostic and academic applications.

Praesto® Pre-Activated Chromatography Resins - FAQs

Principali applicazioni

  • Purificazione delle proteine
  • Production of customized affinity chromatography media

Vantaggi

  • High productivity
  • High capacity
  • Enhanced pressure/flow performance
  • Secure, validated supply and regulatory support

Certificazioni

  • Prodotto in condizioni cGMP

Imballi tipici

  • Bulk Resin
  • Production-Scale OPUS® Columns
  • OPUS® Robocolumns®
  • OPUS® MiniChrom Columns
  • HT Columns

Caratteristiche chimiche e fisiche tipiche:

Struttura del polimero Highly cross linked agarose
Aspetto Spherical beads
Gruppo funzionale CNBr
Stabilità chimica After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents
Distribuzione granulometrica - µm 45, 65, 90 µm
Pressione/flusso (min.) - a 3 bar in una colonna di 2.6 x 20 cm (impaccata a pressione a 4 bar) 200 cm/h
Limite di esclusione per proteine globulari 10 7 Daltons
Stabilità del pH, CIP (a breve termine) 2 - 11
pH di stabilità, range operativo 3 - 11
Conservazione/Stoccaggio consigliato 2 - 8 °C
Conservazione/Stoccaggio consigliato 20% EtoH (after coupling)