Chromalite A860/5952 is ideal for isolation of proteins, especially for proteins from milk and dairy products. The charge on the protein is dependent of the working pH, resulting in three situations:

pH = IEP (iso-electric-point) = Protein is neutral
pH <IEP = Protein is positively charged
pH >IEP = Protein is negatively charged

By selecting the right pH conditions, selective adsorption of a protein can be achieved. Optimal protein separation is achieved when the target protein possesses a charge that is opposite to the other proteins in the solution. Under these conditions, protein will selectively adsorb on an IEX resin. A well-known example for this approach is the separation of Lactoferrin from whey using an anion exchanger. A similar approach can be applied to other proteins. Conditions are adjusted in such a way that the target protein possesses a negative charge, while the untargeted proteins are positively charged.

Chromalite A860/5952 is an ideal choice, due to its hydrophilic and protein-compatible matrix. The resin rigidity and particle size also allows packing in industrial columns.

Principali applicazioni

  • Purificazione delle proteine

Vantaggi

  • Adsorbimento altamente reversibile
  • Eccellente resistenza all'inquinamento organico
  • Efficiente rigenerazione

Certificazioni

  • Compliant with ResAP(2004) 3
  • Halal
  • Kosher
  • esente da TSE BSE OGM

Imballi tipici

  • 50 g
  • 250 g
  • 1 kg
  • 2-10 Kg
  • 25 Kg

Caratteristiche chimiche e fisiche tipiche:

Aspetto Spherical beads
Gruppo funzionale Quaternary Ammonium
Fornito come Wet in Cl- form
Capacità volume (min.) 0.8 eq/L
Capacità peso (min.) 4 eq/kg
Distribuzione granulometrica (90% nel range) 300 - 850 µm
Umidità totale 66 - 72 percento
Typical swelling Cl- → OH- < 25 percento
Limite di stabilità del pH 1 - 14
Condizioni ottimali di conservazione 2 - 20 °C
Forma ionica Cl-
Data di scadenza (dalla data di produzione) 5 anni