To support in the development and manufacture of biopharmaceuticals, Purolite has developed a range of pre-activated agarose resins. These resins enable manufacturers to couple their own ligands to develop affinity chromatography solutions. Epoxy pre-activated resins are available in three particle sizes - 45 μm, 65 μm and 90 μm.

Praesto Epoxy resins have been designed to offer a simple solution for the immobilization of ligands onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D proof of concept to larger scale bioprocess production columns. Praesto Epoxy resins offer the versatility to couple ligands through primary amine, hydroxyl and thiol groups. The Praesto Epoxy resin design incorporates a spacer which separates the ligand from the chromatography carrier enabling maximum efficiency of the ligand. The epoxide group forms a stable linkage between the matrix and ligand, which has very low ligand leakage and high caustic stability. Many well-documented references (published over several years) are publicly available.


Principal Applications

  • Protein purification
  • Production of customized affinity chromatography media


  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Epoxy
Chemical stability After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents
Particle Size - µm 45 µm
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 200 cm/h
Exclusion limit for globular proteins 10 7 Daltons
pH stability, CIP (short term) 2 - 13
pH stability, working range 2 - 13
Recommended Storage 100% IPA - 2 - 8 °C
Recommended Storage 20% EtoH (after coupling)