Highly Cross Linked Pre-Activated Agarose Base Matrix
Praesto Epoxy resins have been designed to offer a simple solution for ligand immobilization onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D to large-scale bioprocess production columns.
Praesto Epoxy resins offer the versatility to couple ligands through primary amine, hydroxyl and thiol groups. The resin design incorporates a spacer which separates the ligand from the chromatography carrier, enabling maximum efficiency of the ligand. The epoxide group forms a stable linkage between the matrix and ligand, which has very low ligand leakage and high caustic stability.
Praesto® Pre-Activated Chromatography Resins - FAQs
|Polymer Structure||Highly cross linked agarose|
|Chemical stability||After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents|
|Particle Size - µm||45, 65, 90 µm|
|Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar)||200 cm/h|
|Exclusion limit for globular proteins||10 7 Daltons|
|pH stability, CIP (short term)||2 - 13|
|pH stability, working range||2 - 13|
|Recommended Storage||100% IPA - 2 - 8 °C|
|Recommended Storage||20% EtoH (after coupling)|