To support in the development and manufacture of biopharmaceuticals, Purolite has developed a range of pre-activated agarose resins. These resins enable manufacturers to couple their own ligands to develop affinity chromatography solutions. CNBr pre-activated chemistries are available in three particle sizes - 45 μm, 65 μm and 90 μm.

Praesto CNBr resins have been designed to offer a simple solution for the immobilization of ligands onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D proof of concept to larger scale bioprocess production columns. The use of Cyanogen bromide (CNBr) pre-activated base matrices is a well-established, rapid and familiar technique for the coupling of bio-specific ligands to generate affinity chromatography purification media. This choice of pre-activation chemistry is well suited for research, diagnostic and academic applications. Coupling is via primary amino groups. Praesto CNBr resins have a cyanogen-active group which form an isourea linkage between ligand and resin. Many well documented references (published over several decades) are publicly available.


Principal Applications

  • Protein purification
  • Production of customized affinity chromatography media


  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group CNBr
Chemical stability After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents
Particle Size - µm 65 µm
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 400 cm/h
Exclusion limit for globular proteins 10 7 Daltons
pH stability, CIP (short term) 2 - 11
pH stability, working range 3 - 11
Recommended Storage 2 - 8 °C
Recommended Storage 20% EtoH (after coupling)