Highly cross linked pre-activated agarose base matrix
To support in the development and manufacture of biopharmaceuticals, Purolite has developed a range of pre-activated agarose resins. These resins enable manufacturers to couple their own ligands to develop affinity chromatography solutions. CNBr pre-activated chemistries are available in three particle sizes - 45 μm, 65 μm and 90 μm.
Praesto CNBr resins have been designed to offer a simple solution for the immobilization of ligands onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D proof of concept to larger scale bioprocess production columns. The use of Cyanogen bromide (CNBr) pre-activated base matrices is a well-established, rapid and familiar technique for the coupling of bio-specific ligands to generate affinity chromatography purification media. This choice of pre-activation chemistry is well suited for research, diagnostic and academic applications. Coupling is via primary amino groups. Praesto CNBr resins have a cyanogen-active group which form an isourea linkage between ligand and resin. Many well documented references (published over several decades) are publicly available.
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|Polymer Structure||Highly cross linked agarose|
|Chemical stability||After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents|
|Particle Size - µm||45 µm|
|Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar)||200 cm/h|
|Exclusion limit for globular proteins||10 7 Daltons|
|pH stability, CIP (short term)||2 - 11|
|pH stability, working range||3 - 11|
|Recommended Storage||2 - 8 °C|
|Recommended Storage||20% EtoH (after coupling)|