Praesto® CNBr resins offer a simple solution for ligand immobilization onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D to large-scale bioprocess production columns.  Purolite Life Sciences can also offer custom solutions through our use of jetting technology, enabling the precise control of any bead size.

The use of Cyanogen bromide (CNBr) pre-activated base matrices is a well-established, rapid and familiar technique for the coupling of bio-specific ligands to generate affinity chromatography purification media. This choice of pre-activation chemistry is well suited for research, diagnostic and academic applications.

Praesto® Pre-Activated Chromatography Resins - FAQs

Principal Applications

  • Protein purification (20-500 kDa)
  • Production of customized affinity chromatography media

Advantages

  • High productivity
  • High capacity
  • Enhanced pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • Bulk Resin
  • Production-Scale OPUS® Columns
  • OPUS® Robocolumns®
  • OPUS® MiniChrom Columns
  • HT Columns

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group CNBr
Chemical stability After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents
Particle Size - µm 45, 65, 90 µm
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 200 cm/h
Exclusion limit for globular proteins 10 7 Daltons
pH stability, CIP (short term) 2 - 11
pH stability, working range 3 - 11
Recommended Storage 2 - 8 °C
Recommended Storage 20% EtoH (after coupling)

The statements, technical information and recommendations contained herein are believed to be accurate as of the date hereof. Since the conditions and methods of use of the product and of the information referred to herein are beyond our control, Purolite expressly disclaims any and all liability as to any results obtained or arising from any use of the product or reliance on such information; NO WARRANTY OF FITNESS FOR ANY PARTICULAR PURPOSE, WARRANTY OF MERCHANTABILITY OR ANY OTHER WARRANTY, EXPRESSED OR IMPLIED, IS MADE CONCERNING THE GOODS DESCRIBED OR THE INFORMATION PROVIDED HEREIN. The information provided herein relates only to the specific product designated and may not be applicable when such product is used in combination with other materials or in any process. Nothing contained herein constitutes a license to practice under any patent and it should not be construed as an inducement to infringe any patent and the user is advised to take appropriate steps to be sure that any proposed use of the product will not result in patent infringement.