Highly Cross Linked Pre-Activated Agarose Base Matrix
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Praesto® CNBr resins offer a simple solution for ligand immobilization onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D to large-scale bioprocess production columns. Purolite Life Sciences can also offer custom solutions through our use of jetting technology, enabling the precise control of any bead size.The use of Cyanogen bromide (CNBr) pre-activated base matrices is a well-established, rapid and familiar technique for the coupling of bio-specific ligands to generate affinity chromatography purification media. This choice of pre-activation chemistry is well suited for research, diagnostic and academic applications.
|Polymer Structure||Highly cross linked agarose|
|Chemical stability||After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents|
|Particle Size - µm||45, 65, 90 µm|
|Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar)||200 cm/h|
|Exclusion limit for globular proteins||10 7 Daltons|
|pH stability, CIP (short term)||2 - 11|
|pH stability, working range||3 - 11|
|Recommended Storage||2 - 8 °C|
|Recommended Storage||20% EtoH (after coupling)|
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