CalB immo 8285 was jointly developed and is co-marketed by c-LEcta and Purolite.
Immobilized, recombinant Lipase B from Candida antarctica (CalB) produced by microbial fermentation with Pichia pastoris. CalB immo 8285 is covalently immobilized and is suitable for applications in water, organic solvents as well as solvent-free systems and can be used for (regio- and stereoselective) hydrolysis, esterifications and transesterifications. The lipase is immobilized by covalent immobilization onto Purolite’s highly hydrophobic carrier Purolite ECR8285 (an epoxy/butyl methacrylate co-polymer).
CalB Immo 8285 has many advantages compared to similar commercial preparations:
- high activity
- possibility to use in aqueous, organic and bi-phasic systems
- security of supply from kg to ton scale
- full regulatory support

Principal Applications

  • Esterifications (regio- and stereo-selective)
  • Transesterfication
  • Amidation
  • Fats and oils modification
  • Hydrolysis of esters


  • Covalent immobilization
  • Suitable for applications in aqueous systems and organic solvents
  • High enzyme activity (>10000PLU/g)
  • Security of supply from Kg to ton scale
  • Full regulatory support

Regulatory Approvals

  • Animal-free statement (TSE/BSE-free certificate)
  • TSE/BSE/GMO free
  • Council of Europe Resolution ResAP (2004)6
  • Compliant with FDA regulation 21 CFR 173.65
  • Halal
  • Kosher in 2018

Typical Packaging

  • 10g
  • 50g
  • 250 g
  • 1Kg

Typical Physical & Chemical Characteristics:

Appearance White to slightly yellow spherical beads
Supplied as Dry
Particle Size Range 250 - 1000 µm
Total moisture 0 - 5 %
Enzyme activity (min.) 10000 PLU/gdry
Expiry date (from date of manufacture) 1 year