Praesto RoboColumns® are designed for easy process development and parameter screening, as well as for small scale purification or sample preparation. Pre-packed columns provide a small bed volume for fast results and minimal sample and buffer consumption, offering both very high reproducibility and very good scalability. The RoboColumn® format is designed for use with robotic workstations for HTPD work. Praesto RoboColumns® are packed by Atoll GmbH and delivered ready for use. They are 100% quality checked for HETP and asymmetry. Individual laser labelling ensures traceability to corresponding resin and column performance data. The columns are made of biocompatible polypropylene. Praesto APc is a Protein A resin designed on a novel high flow agarose backbone, which addresses today s early-phase clinical trial challenges for high productivity together with cost-effective MAb capture. It is purpose-designed and evaluated for phase I and II clinical trials, where typically less than 20 reuse cycles are performed. Alternatively Praesto APc can be implemented across all phases due to long life time. Before PIII, for innovator therapies, Purolite Life Sciences recommends you then switch from Praesto APc to Praesto AP, since they both utilize the same Protein A ligand species.


Principal Applications

  • Protein purification
  • MAb Purification


  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • One Row of 8 prepacked 200µl columns

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Protein A
Inner diameter 5 mm
Packed bed height 10 mm
Column volume 200 µl
Connector for use with liquid robotic handling stations
Column material Polypropylene
Bed support Filter
Chemical stability All commonly used aqueous buffers, pH 1-14, organic solvents
Avoid Halogenated organic solvents, hexane
Working pressure (max.) 8 bar
Particle Size - µm 85 µm
Dynamic Binding Capacity (min.)- 3 minutes residence time 25 hlgG/ml
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column 500 cm/h
pH stability, CIP (short term) 2 - 12
pH stability, working range 3 - 10
Recommended Storage 2 - 8 °C
Recommended Storage 20% ethanol