Praesto MiniChrom columns are designed for process development and parameter screening, as well as for small scale purification or sample preparation. Minichrom 8x20mm columns have a 2 cm bed height and a resin volume of 1 mL. MiniChrom columns are packed by Atoll GmbH and delivered ready for use. They are 100% quality checked for HETP and asymmetry. Individual laser labelling ensures traceability to corresponding resin and column performance data. The columns are made of biocompatible polypropylene. Praesto MiniChrom 8x20mm pre-packed columns provide a small bed volume for fast results and minimal sample and buffer consumption, as well as convenience in media screening and easy, direct connection to most chromatography systems Praesto AP is a Protein-A resin designed on a novel high flow agarose backbone which uses a recombinant alkaline-tolerant Protein-A ligand expressed in E.coli. The Protein-A ligand used for Praesto AP resulted from protein engineering of one of the IgG-binding domains of Protein-A. In particular, the amino acids sensitive to alkali conditions were identified and then substituted with more stable ones to deliver superior alkaline stability which provides hundreds of re-cycles. Praesto AP addresses today s late stage clinical manufacturing challenges for high productivity together with cost-efficient MAb capture. It is purpose-designed and evaluated for the production of late phase clinical trial material, PIII and commercial manufacture, where typically hundreds of reuse cycles are performed.


Principal Applications

  • Protein purification
  • MAb Purification


  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • Prepacked 1ml column

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Protein A
Inner diameter 8 mm
Packed bed height 20 mm
Column volume 1 ml
Connector fingertight 1/16", female
Column material Polypropylene
Bed support Filter
Chemical stability All commonly used aqueous buffers, pH 1-14, organic solvents
Avoid Halogenated organic solvents, hexane
Working pressure (max.) 30 bar
Particle Size - µm 85 µm
Dynamic Binding Capacity (min.)- 3 minutes residence time 25 hlgG/ml
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column 500 cm/h
pH stability, CIP (short term) 2 - 12
pH stability, working range 3 - 10
Recommended Storage 2 - 8 °C
Recommended Storage 20% ethanol