Praesto MiniChrom columns are designed for process development and parameter screening, as well as for small scale purification or sample preparation. MiniChrom columns are packed by Atoll GmbH and delivered ready for use. They are 100% quality checked for HETP and asymmetry. Individual laser labelling ensures traceability to corresponding resin and column performance data. The columns are made of biocompatible polypropylene. Praesto MiniChrom pre-packed columns provide a small bed volume for fast results and minimal sample and buffer consumption, as well as convenience in media screening and easy, direct connection to most chromatography systems. Praesto AC is a Protein A resin designed to address today's clinical manufacturing challenges for high productivity, cost-effective MAb capture. Praesto AC combines good capacity, excellent pressure/flow performance and NaOH CIP stability for up to 20 cycles, meeting the common requirements for production of materials for PI and PII clinical trials. Praesto AC can also be used in small scale MAb purification, purification of MAbs for diagnostics, in process development and in pre-clinical processes. Praesto AP is a Protein-A resin designed on a novel high flow agarose backbone which uses a recombinant alkaline-tolerant Protein-A ligand expressed in E.coli. The Protein-A ligand used for Praesto AP resulted from protein engineering of one of the IgG-binding domains of Protein-A. In particular, the amino acids sensitive to alkali conditions were identified and then substituted with more stable ones to deliver superior alkaline stability which provides hundreds of re-cycles. Praesto AP addresses today s late stage clinical manufacturing challenges for high productivity together with cost-efficient MAb capture. It is purpose-designed and evaluated for the production of late phase clinical trial material, PIII and commercial manufacture, where typically hundreds of reuse cycles are performed.


Principal Applications

  • Protein purification
  • MAb Purification


  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • Prepacked 5ml chromatography column

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Protein A
Inner diameter 8 mm
Packed bed height 100 mm
Column volume 5 ml
Connector fingertight 1/16", female
Column material Polypropylene
Bed support Filter
Chemical stability All commonly used aqueous buffers, pH 1-14, organic solvents
Avoid Halogenated organic solvents, hexane
Working pressure (max.) 30 bar
Particle Size - µm 85 µm
Dynamic Binding Capacity (min.)- 3 minutes residence time 25 hlgG/ml
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column 500 cm/h
pH stability, CIP (short term) 2 - 12
pH stability, working range 3 - 10
Recommended Storage 2 - 8 °C
Recommended Storage 20% ethanol