What is Seeding?

Seeding is the manfacturing method of producing chromatographic resin beads that are mono-size and ultra-fine. The beads produced have a narrow particle size distribution and are usually between 5 - 50 μm in size, although in some cases seeding can be used to produce larger beads. 

Polystyrene 'seeds' are first produced through dispersion polymerization of styrene in alcohols.  These initial seeds are in the range of 1 - 5 µm.

'Seeds' can be grown in two ways depending on the physical properties required in the final product:

Non-porous beads are swelled with monomers only

Macroporous beads are swelled with a combination of monomers and porogens

Advantages of Seeding

As the beads produced are uniform in size this results in improved performance in areas such as:

  • Resolution (efficiency & selectivity)
  • Loading capacity
  • Back pressure/speed (size, porosity)
     
PRODUCTS PRODUCED BY SEEDING TECHNOLOGY

Chromalite
® GN resins for calibration and HPLC

Chromalite® MN and AD resins for Solid Phase Extraction and Reversed Phase Chromatography

Microscopic image of Chromalite resin produced by seeding technology
Microscopic image of Chromalite resin produced by seeding technology

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