The Praesto® Range

Purolite Life Sciences offer an extensive range of chromatography resins on various base matrices.  Covering capture and polishing techniques with affinity Protein A and ion exchange resins on a high flow agarose base matrix for the purification of large, complex biologics such as monoclonal antibodies. 

The Praesto® range includes a selection of affinity chromatography and ion exchange chromatography agarose resins, delivering exceptional results from capture to high-resolution polishing steps.

All Praesto® products provide an advanced, high-flow, highly cross-linked agarose base matrix, and are available in a range of formats to suit your process needs, from high throughput to full-scale commercial manufacture.  Process development and up-scaling is streamlined by using our pre-packed and pre-qualified formats.

The porosity and pore structures are designed specifically for the purification of monoclonal antibodies.

 

To discuss how we can assist your journey to process excellence, contact a specialist

​Why agarose?

Agarose is widely considered the best material available for protein purification resins, with the vast majority of approved commercial processes today utilising agarose in their purification platform.

Compared to synthetic resins, agarose resins provide: 

  • Easy handling at large scales (packing and re-packing)
  • Exceptional lifetime due to NaOH stability
  • Extremely low non-specific binding (due to naturally hydrophilic properties)

​Monoclonal Antibody Platform Purification

The purification of commercially-available monoclonal antibodies (MAbs) on the market today is typically done in three chromatography steps:

1) Utilise Protein-A affinity resins - typically the first choice in the purification process because they deliver high purity (> 99%) and yield in a single step.

2) SP cation exchange as the first polishing step in bind and elute mode to remove aggregates and HCP

3)  Q anion exchange in flow-through mode, as a scavenger to remove trace contaminates and ensure sufficient viral clearance.
 

Praesto® Products by Application

Ion Exchange Chromatography

Protein A Chromatography