Protein and MAb Purification Resins
The Praesto® range is a modern range of high-performing chromatography resins that deliver exceptional results on a high flow agarose base matrix with a porosity and pore structures ideal for the purification of large, complex biologics such as monoclonal antibodies.
MONOCLONAL ANTIBODY PURIFICATION PLATFORM
The purification of commercially-available monoclonal antibodies (mAbs) on the market today is typically done in three chromatography steps:
1) Utilise Protein-A affinity resins - typically the first choice in the purification process because they deliver high purity (> 99%) and yield in a single step.
2) SP cation exchange as the first polishing step in bind and elute mode to remove aggregates and HCP
3) Q anion exchange in flow-through mode, as a scavenger to remove trace contaminates and ensure sufficient viral clearance.
How do Protein and MAb Purification Resins work?
Affinity chromatography is a method of separating proteins based on a highly specific, reversible interaction between an individual or group of proteins, and a suitable ligand attached to a chromatographic matrix.
Ion Exchange chromatography is used to separate molecules based on their net surface charge.
Anion exchange chromatography uses a positively charged ion exchange resin with an affinity for molecules having net negative surface charges.
Cation exchange chromatography uses a negatively charged ion exchange resin with an affinity for molecules having net positive surface charges.