What Is Jetting?

Purolite Life Sciences are the only resin supplier in the world with the ability to manufacture process-scale volumes of both copolymer and agarose resin beads with uniform particles sizes. The more uniform your resin beads, the more consistently your chromatographic process will perform. 

Our technology is called 'Jetting' - a patented, continuous manufacturing process for producing chromatographic resin beads with a narrow, uniform particle size distribution.


From a manufacturing perspective, our non-solvent Jetting process gives us yields of 100%. Compare this with the traditional batch emulsification method of resin manufacture - which does use solvents - and yields can be as low as 40%. This means that Jetted products reduce product wastage by 60%, whilst reducing our environmental impact considerably.

Jetting is a more streamlined, efficient manufacturing method, which eliminates the need for a time consuming screening process. This reduces manufacturing time in our facility, reducing lead times considerably and enabling us to pass those savings on to our customers.


ADVANTAGES

  • No fines =
    • Higher flow velocities
    • Improved column packing efficiency, reproducibility and stability
    • No column net clogging
    • Reduced fouling = longer lifetime
    • More consistent packing characteristics
    • Lower back pressure
    • Possible to use larger mesh size column filters/nets
    • Less risk for fouling of resin and/or supports
    • Improved lifetime
  • Faster mass transfer kinetics, shorter processing time frames
  • Improved resolution, more concentrated elutions
  • Increased efficiency & productivity 
  • Non-solvent = Greener manufacturing
  • Precise lot-to-lot reproducibility

Fig. 1 Continuous Membrane Jetting Technology

Jetting technology operates with two different processes, depending on the particle size needed. The first Purolite jetting process – membrane or ‘can’ jetting – entails using a membrane with very small, discrete orifices, through which the polymer is passed. This device is capable of producing uniform particle size chromatographic resins from ~15 to 250 micron.  Cooled, and then hardened, the beads are then collected in a vessel.  The process can run continuously.

Subsequent process steps (cross-linking, derivatization) are similar to those for emulisified beads.

This process is unique to Purolite, and an innovation in the manufacture of agarose beads.  There are significant opportunities to adjust the process to account for variability in regards to incoming materials, to ensure a highly consistent product with respect to particle size distribution.

The second process, plate jetting, utilises a plate with many precisely engineered holes, through which the resin polymer is passed. Uniform particle size chromatographic resins of ~150 to >1,000 micron can be produced using this method.

Jetting technology is used to manufacture select products in our ranges of agarose and synthetic chromatography resins, including Praesto® Jetted A50, Praesto® Jetted Q35, Praesto® Jetted SP35 and Chromalite®.

Figure 2: Purolites 'Jetted' uniform beads and the market leading resin