A Real World Example
All previous data sets have been for illustrative purposes only, but a real-world example of this is the comparison of Praesto® Jetted A50 (a 50 µm bead) and MabSelect™ PrismA (a 60 µm bead), as shown in Figures 9 and 10 respectively. Measurements were taken via optical microscopy, with a minimum bead count of 2500 particles per measurement and bin sizes of 0.25 micron.
When comparing only the volume distribution there is minimal difference in the particle size distribution of Praesto® Jetted A50 and MabSelect™PrismA. However, there is stark contrast in the number distribution of the two resins.
Praesto® Jetted A50, which is made using Purolite’s proprietary Jetting technology, has no fines and very little course material, therefore it does not require screening. It is a uniform particle size 50 µm bead with an average particle size distribution of 95% within 35 - 90 µm. MabSelect™PrismA is a 60 µm bead, with an average particle size distribution of 95% within 20 -100 µm.
In the tested sample of MabSelect™PrismA, which is produced by a batch wise emulsification technology, approximately 25% of all particles present are smaller than 25 microns, which can block column nets and the interstitial voids of a packed bed.