The Use of Resin Technology in Bioprocessing

A bioprocess is any process that uses complete living cells or their components (e.g., bacteria, enzymes, chloroplast) to obtain desired products. This process is commonly referred to as fermentation.

Resin technology is used to chromatographically separate and purify the targeted product in the fermentation broth. Large molecules such as proteins, enzymes, and antibodies are highly sensitive to fluctuations in their environment such as temperature, pH, salt concentration, interaction with other molecules and the system, strain of the flow etc… therefore the chromatography step needs to be as gentle as possible during the loading and the elution process. This requires resin that is much more hydrophilic in nature. If the resin is too hydrophobic such as polystyrenic based resin, significant non-specific binding occurs where proteins can denature and low yield can result.

More hydrophilic resins are offered for such applications with different base matrices.

Protein Purification with polymethacrylic resins: These resins are highly porous to allow very large molecules such as proteins to be extracted. The hydrophilic behavior of the polymethacrylate will be gentle on the protein to prevent any unnecessary loss of activity and yield. These resins can be found under the Chromalite brand M series products. Polymethacrylates are highly rigid polymers where fast flow or deep resin bed can be used without any significant bed compression for higher productivity.

Antibody and Protein Purification with agarose resins: These are the most hydrophilic resins in the market.  The activity and protein yield using an agarose matrix is expected to be better than using a polymethacrylate. The capacity within a certain resin bed volume is also expected to be higher. There is no expected non specific binding to occur but the resin is softer than the polymethcrylate and will require shallow bed column during operations.