Case Study for CalB immo Plus Immobilized Enzyme

In addition to a complete range of enzyme carriers - Lifetech™ ECR, the Purolite team has developed ready-to-use immobilized enzymes for a variety of applications in industry. The immobilized enzymes developed at Purolite cover different groups such as transferases, hydrolases, isomerases,etc

CalB immo Plus is one success story involving the joint development of an immobilized enzyme with c-Lecta. CalB immo Plus is an immobilized, recombinant Lipase B from Candida antarctica (CalB), produced by microbial fermentation with Pichia pastoris, immobilized by adsorption on a robust Lifetech™ ECR resin (ECR1030M, a DVB/methacrylate co-polymer). This immobilized enzyme finds many different applications in industry, as presented in Figure 1.

Figure 1. Overview of possible reactions and media catalysed by CalB

Figure 3. Operational recyclability of CalB immo Plus

 

The CalB immo Plus provides similar, or better, esterification and amidation enzyme activity versus a leading competitor product.

The synergy between a robust recombinant over-expression system with an outstanding polymer for enzyme adsorption makes CalB
immo Plus a superior product; outperforming other commercial preparations.

Lifetech™ ECR resins lead the market in a range of available porosities, particle sizes, functional groups and chemistries. Immobilized enzymes on Lifetech™ ECR resins expand the biocatalysis portfolio, offering robust, cost-effective and efficient ready-to-use biocatalysts.