Overview

Mechanical Stability

Lifetech™ ECR resins show outstanding mechanical stability compared to other commercial resins. Mechanical stability is measured by magnetic stirring tests. In this example, resin is added to demineralized water in a glass beaker and mixed using a magnetic stirrer for a period of 3 hours at room temperature. Samples from the solution were analyzed at various time intervals and the absorbance measured at 400 nm. The increase in the absorbance is related to the formation of particulates in the water due to the resin beads breaking.

Below, a comparison between a methacrylate from Purolite® and two commercially available epoxy methacrylates (competitor A&B) can be seen.

Mechanical stability of Lifetech™ ECR resins for enzyme adsorption (click to enlarge)
Mechanical stability of Lifetech™ ECR for covalent enzyme immobilization (click to enlarge)
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Industry Example

Using Lifetech™ ECR1030M robust divinyl benzene/methacrylate polymer for immobilzation of lipase CalB.

Candida antarctica lipase B (CalB) is the most versatile biocatalyst and is widely used in industry. Its advantages range from thermostability, stereoselectivity and activity in organic solvents to the acceptance of nucleophiles other than water. The actual standard for industrial applications of CalB is the immobilized enzyme on Lewatit® VP OC 1600 and is marketed under the name Novozym® 435.

A new methacrylic enzyme carrier (Lifetech™ ECR1030M) for the immobilization by adsorption of CalB and for use in batch or column reactors has been developed. The resin allows a typical activity of > 10000 PLU/gdry, which is similar to Novozym® 435. With a protein loading of 24.4 mg/gwet it is possible to obtain immobilization yields higher than 90%. Unlike the resin used for the production of Novozym® 435, Lifetech™ ECR1030M possesses high mechanical stability and is therefore more suitable for applications that expose the polymers to high mechanical stress, such as multiple reuses in stirred reactors.

Lipase CalB immobilized on Lifetech™ ECR1030M shows excellent performance in transesterification of vegetable oil and shows the same kinetics as Lipozyme® 435.

The graph shows the initial testing of CalB preparation immobilized on carriers for the transesterification of vegetable oil.