Protein Ion Exchange Chromatography

Protein chromatography is a powerful, scalable method used to isolate a targeted protein of interest from a more complex mixture. Designed with a range of particle sizes, functionalities and base matrices suitable for lab to process-scale applications, our resins help you to achieve optimal yields and product purity throughout process scale-up.

For protein ion exchange chromatography, we have a range of chemistries functionalized onto Agarose and Methacrylic polymers to suit your process requirements. The reasons for using these two matrices is to avoid any non-specific binding that is typically prevalent with the polystyrenic resins we also offer.

Proteins include several types of molecules such as enzymes, peptides, and even oligonucleotides. These proteins are usually extracted after a fermentation process. Currently, chromatographic methods represent the most efficient way to isolate enzymes in high quality and purity and by preserving their activity.

Using ion exchange (IEX) chromatography, different types of enzymes are separated based on their net charge. This method can operate under mild conditions, thereby preserving the normal conformation of the protein sample.