Core Technologies

As the global leader in resin technology, we develop and manufacture small beads that are used in the most regulated industries in the world to separate, remove or recover very specific elements and compounds.

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Healthcare & Life Sciences

With 40 years of manufacturing expertise and 30 years of regulatory experience, we supply leading separation, purification and extraction technologies to support chromatography and biocatalysis applications in healthcare and life sciences.

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Biopharmaceuticals and Antibody Processing
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Analytical Lab Testing and Screening
Overview Affinity Chromatography HIS-TAG Biocatalysis Screening Ligand Addition Monoclonal Antibody Purification Kits Protein Ion Exchange Chromatography Reference / Calibration Reversed Phase Chromatography

Purolite Corporation

We are the world leader in resin-based separation, purification and extraction technology, that provides sustainable solutions for our environment, businesses and healthcare.

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Overview Steve Brodie Don Brodie Jacob Brodie Howard Brodie
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Protein Ion Exchange Chromatography / Analytical Lab Testing and Screening / Healthcare & Life Sciences / purolite

Protein Ion Exchange Chromatography

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Nos équipes de support technique et nos experts sont toujours prêts pour vous assistez. Purolite est à votre disposition

Overview

Protein Ion Exchange Chromatography

Protein chromatography is a powerful, scalable method used to isolate a targeted protein of interest from a more complex mixture. Designed with a range of particle sizes, functionalities and base matrices suitable for lab to process-scale applications, our resins help you to achieve optimal yields and product purity throughout process scale-up.

For protein ion exchange chromatography, we have a range of chemistries functionalized onto Agarose and Methacrylic polymers to suit your process requirements. The reasons for using these two matrices is to avoid any non-specific binding that is typically prevalent with the polystyrenic resins we also offer.

Proteins include several types of molecules such as enzymes, peptides, and even oligonucleotides. These proteins are usually extracted after a fermentation process. Currently, chromatographic methods represent the most efficient way to isolate enzymes in high quality and purity and by preserving their activity.

Using ion exchange (IEX) chromatography, different types of enzymes are separated based on their net charge. This method can operate under mild conditions, thereby preserving the normal conformation of the protein sample.

Protein Ion Exchange Chromatography Products by Application

Anion Exchange Chromatography - Agarose Base

Jetted Q35

Agarose Taux de réticulation élevé Strong Anion Exchanger, Uniform Particle Size, 35 µm

Q45

Agarose Taux de réticulation élevé Strong Anion Exchanger, 45 µm particle size

Q65

Agarose Taux de réticulation élevé Strong Anion Exchanger, 65 µm particle size

Q90

Agarose Taux de réticulation élevé Strong Anion Exchanger, 90 µm particle size

Anion Exchange Chromatography - Polymer Base

MDEA/C

Diethylamino Methacrylate Macroporous Weak Anion Exchanger, 100 - 300 µm

MDEA/F

Diethylamino Methacrylate Macroporous Weak Anion Exchanger, 40 - 90 µm

MDEA/M

Diethylamino Methacrylate Macroporous Weak Anion Exchanger, 75 - 125 µm

MQ/C

Quaternary Ammonium Methacrylate Macroporous Strong Anion Exchanger, 100 - 300 µm

MQ/F

Quaternary Ammonium Methacrylate Macroporous Strong Anion Exchanger, 40 - 90 µm

MQ/M

Quaternary Ammonium Methacrylate Macroporous Strong Anion Exchanger, 75 - 125 µm

Cation Exchange Chromatography - Agarose Base

Jetted SP35

Agarose Taux de réticulation élevé Strong Cation Exchanger, Uniform Particle Size, 35 µm

SP200

Agarose Taux de réticulation élevé Strong Cation Exchanger, 200 µm particle size

SP45

Agarose Taux de réticulation élevé Strong Cation Exchanger, 45 µm particle size

SP65

Agarose Taux de réticulation élevé Strong Cation Exchanger, 65 µm particle size

SP90

Agarose Taux de réticulation élevé Strong Cation Exchanger, 90 µm particle size

Cation Exchange Chromatography - Polymer Base

MCM/C

Carboxymethyl Methacrylate Macroporous Weak Cation Exchanger, 100 - 300 µm

MCM/F

Carboxymethyl Methacrylate Macroporous Weak Cation Exchanger, 40 - 90 µm

MCM/M

Carboxymethyl Methacrylate Macroporous Weak Cation Exchanger, 75 - 125 µm

MS/C

Sulfopropyl Methacrylate Macroporous Strong Cation Exchanger, 100 - 300 µm

MS/F

Sulfopropyl Methacrylate Macroporous Strong Cation Exchanger, 40 - 90 µm

MS/M

Sulfopropyl Methacrylate Macroporous Strong Cation Exchanger, 75 - 125 µm