Overview

Protein Ion Exchange Chromatography

Protein chromatography is a powerful, scalable method used to isolate a targeted protein of interest from a more complex mixture. Designed with a range of particle sizes, functionalities and base matrices suitable for lab to process-scale applications, our resins help you to achieve optimal yields and product purity throughout process scale-up.

For protein ion exchange chromatography, we have a range of chemistries functionalized onto Agarose and Methacrylic polymers to suit your process requirements. The reasons for using these two matrices is to avoid any non-specific binding that is typically prevalent with the polystyrenic resins we also offer.

Proteins include several types of molecules such as enzymes, peptides, and even oligonucleotides. These proteins are usually extracted after a fermentation process. Currently, chromatographic methods represent the most efficient way to isolate enzymes in high quality and purity and by preserving their activity.

Using ion exchange (IEX) chromatography, different types of enzymes are separated based on their net charge. This method can operate under mild conditions, thereby preserving the normal conformation of the protein sample.

Protein Ion Exchange Chromatography | Productos por Aplicación

Anion Exchange Chromatography - Agarose Base

Jetted Q35

Agarose Alto crosslink Intercambiador de aniones fuertes, Uniform Particle Size, 35 µm

Q45

Agarose Alto crosslink Intercambiador de aniones fuertes, 45 µm particle size

Q65

Agarose Alto crosslink Intercambiador de aniones fuertes, 65 µm particle size

Q90

Agarose Alto crosslink Intercambiador de aniones fuertes, 90 µm particle size

Anion Exchange Chromatography - Polymer Base

MDEA/C

Diethylamino Methacrylate Macroporoso Weak Anion Exchanger, 100 - 300 µm

MDEA/M

Diethylamino Methacrylate Macroporoso Weak Anion Exchanger, 75 - 125 µm

MQ/C

Quaternary Ammonium Methacrylate Macroporoso Intercambiador de aniones fuertes, 100 - 300 µm

MQ/M

Quaternary Ammonium Methacrylate Macroporoso Intercambiador de aniones fuertes, 75 - 125 µm

Cation Exchange Chromatography - Agarose Base

Jetted SP35

Agarose Alto crosslink Intercambiador de cationes fuertes, Uniform Particle Size, 35 µm

SP200

Agarose Alto crosslink Intercambiador de cationes fuertes, 200 µm particle size

SP45

Agarose Alto crosslink Intercambiador de cationes fuertes, 45 µm particle size

SP65

Agarose Alto crosslink Intercambiador de cationes fuertes, 65 µm particle size

SP90

Agarose Alto crosslink Intercambiador de cationes fuertes, 90 µm particle size

Cromatografía de intercambio catiónico

MS/C

Sulfopropyl Methacrylate Macroporoso Intercambiador de cationes fuertes, 100 - 300 µm

MS/M

Sulfopropyl Methacrylate Macroporoso Intercambiador de cationes fuertes, 75 - 125 µm

Bioprocessing

Monoclonal antibody and recombinant protein purification

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Protein Ion Exchange Chromatography

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Overview

Protein Ion Exchange Chromatography

Protein Ion Exchange Chromatography | Productos por Aplicación