Core Technologies

As the global leader in resin technology, we develop and manufacture small beads that are used in the most regulated industries in the world to separate, remove or recover very specific elements and compounds.

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Product Types
Product Types
Adsorbents
Adsorbents
Overview Adsorbents for Enzyme Immobilization Adsorbents for Gases and Organic Vapors Adsorbents of Organic Pollutants Citrus Juice Debittering Corn Sweetener Refining Inorganic Salt Separation Using Adsorbents Patulin Removal Polyphenol Recovery
Catalyst Chelation Resins Chromatographic Separation Resins Fine Mesh Resins Inert Spacer Resins Mixed Bed Resins
Mixed Bed Resins
Overview Mixed Bed Nuclear Mixed Bed Ultrapure Water
Strong Acid Cation Resin Strong Base Anion Resin Weak Acid Cation Resin
Weak Acid Cation Resin
Overview Weak Acid Cation Macroporous Weak Acid Cation Porous
Weak Base Anion Resin
Weak Base Anion Resin
Overview Weak Base Anion Gel Weak Base Anion Macroporous
Industries
Industries
Chemical Processing and Petrochemical Fine Chemicals
Fine Chemicals
Overview Cosmetics Fragrances
Food and Beverage
Food and Beverage
Overview Dairy Flavors Food Ingredients Fruit Juice
Fruit Juice
Overview Citrus Juice Debittering Patulin Removal Polyphenolic Compound Recovery
Gelatin and Collagen Organic Acid Purification Sugar
Sugar
Overview Beet Sugar Refining Cane Sugar Refining
Sweetener
Sweetener
Overview Corn Sweetener Refining
Additional Food and Beverage
Hydrometallurgy Metals Plating Oil & Gas Potable and Groundwater
Potable and Groundwater
Overview Arsenic Nitrate PFAS TOC, Bromide and DBP
Power
Power
Overview Fossil Nuclear
Pulp & Paper Semiconductor - Electronics
Applications
Applications
Adsorption Catalysis Condensate Polishing Demineralization Metal Removal and Recovery Organics Removal Softening

Healthcare & Life Sciences

With 40 years of manufacturing expertise and 30 years of regulatory experience, we supply leading separation, purification and extraction technologies to support chromatography and biocatalysis applications in healthcare and life sciences. 

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Biopharmaceuticals and Antibody Processing
Biopharmaceuticals and Antibody Processing
Overview Antibody Purification Protein Purification Vaccine Production
Pharmaceutical
Pharmaceutical
Overview APIs/Excipients Biocatalysis Milling Media Oligonucleotide Purification Peptide Purification Plasma Fractionation Radionuclide Separation
Medical Devices & Diagnostics
Medical Devices & Diagnostics
Overview Biofluid Treatment Diagnostics Enteral Nutrition
Nutraceuticals
Nutraceuticals
Overview Amino Acid Production Cannabinoid Purification Dairy Protein Isolation Protein Purification Supplement Ingredients Vitamin Purification
Analytical Lab Testing and Screening
Analytical Lab Testing and Screening
Overview Affinity Chromatography HIS-TAG Biocatalysis Screening Ligand Addition Monoclonal Antibody Purification Kits Protein Ion Exchange Chromatography Reference / Calibration Reversed Phase Chromatography

Purolite

We are the world leader in resin-based separation, purification and extraction technology, that provides sustainable solutions for our environment, businesses and healthcare.

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About
About
About Us
About Us
Overview History
Locations Services Manufacturing Customer Support Research & Development Brands
Brands
Chromalite™ CriticalResin™ LifeTech™ Macronet™ Microlite® Praesto® Purophase™ Purofine® Puromet™ Puromill™ Puropack® PuroSorb™ Shallow Shell™ UltraClean™
Environmental Commitment Careers
Resources
Resources
General Information Certifications
Certifications
Product Certifications Quality, Environment, Health and Safety
Terms of Sale Glossary and Definitions Case Studies
News & Events
News & Events
News Events Blog Webinars
Tools
Tools
Purolite Resin System Modeling (PRSM™) Unit Conversion Calculator
Protein Ion Exchange Chromatography / Analytical Lab Testing and Screening / Healthcare & Life Sciences / purolite
Overview

Protein Ion Exchange Chromatography

Protein chromatography is a powerful, scalable method used to isolate a targeted protein of interest from a more complex mixture. Designed with a range of particle sizes, functionalities and base matrices suitable for lab to process-scale applications, our resins help you to achieve optimal yields and product purity throughout process scale-up.

For protein ion exchange chromatography, we have a range of chemistries functionalized onto Agarose and Methacrylic polymers to suit your process requirements. The reasons for using these two matrices is to avoid any non-specific binding that is typically prevalent with the polystyrenic resins we also offer.

Proteins include several types of molecules such as enzymes, peptides, and even oligonucleotides. These proteins are usually extracted after a fermentation process. Currently, chromatographic methods represent the most efficient way to isolate enzymes in high quality and purity and by preserving their activity.

Using ion exchange (IEX) chromatography, different types of enzymes are separated based on their net charge. This method can operate under mild conditions, thereby preserving the normal conformation of the protein sample.

Protein Ion Exchange Chromatography | Produkte nach Anwendung

Anion Exchange Chromatography - Agarose Base

Jetted Q35

Agarose hoch quervernetzt stark basischer Anionenaustauscher, Uniform Particle Size, 35 µm

Q45

Agarose hoch quervernetzt stark basischer Anionenaustauscher, 45 µm particle size

Q65

Agarose hoch quervernetzt stark basischer Anionenaustauscher, 65 µm particle size

Q90

Agarose hoch quervernetzt stark basischer Anionenaustauscher, 90 µm particle size

Anion Exchange Chromatography - Polymer Base

MDEA/C

Diethylamino Methacrylate makroporös Weak Anion Exchanger, 100 - 300 µm

MDEA/M

Diethylamino Methacrylate makroporös Weak Anion Exchanger, 75 - 125 µm

MQ/C

Quaternary Ammonium Methacrylate makroporös stark basischer Anionenaustauscher, 100 - 300 µm

MQ/M

Quaternary Ammonium Methacrylate makroporös stark basischer Anionenaustauscher, 75 - 125 µm

Cation Exchange Chromatography - Agarose Base

Jetted SP35

Agarose hoch quervernetzt stark saurer Kationenaustauscher, Uniform Particle Size, 35 µm

SP200

Agarose hoch quervernetzt stark saurer Kationenaustauscher, 200 µm particle size

SP45

Agarose hoch quervernetzt stark saurer Kationenaustauscher, 45 µm particle size

SP65

Agarose hoch quervernetzt stark saurer Kationenaustauscher, 65 µm particle size

SP90

Agarose hoch quervernetzt stark saurer Kationenaustauscher, 90 µm particle size

Kationen-Austausch Chromatographie

MCM/C

Carboxymethyl Methacrylate makroporös Weak Cation Exchanger, 100 - 300 µm

MCM/M

Carboxymethyl Methacrylate makroporös Weak Cation Exchanger, 75 - 125 µm

MS/C

Sulfopropyl Methacrylate makroporös stark saurer Kationenaustauscher, 100 - 300 µm

MS/M

Sulfopropyl Methacrylate makroporös stark saurer Kationenaustauscher, 75 - 125 µm