Praesto RoboColumns® are designed for easy process development and parameter screening, as well as for small scale purification or sample preparation. Pre-packed columns provide a small bed volume for fast results and minimal sample and buffer consumption, offering both very high reproducibility and very good scalability. The RoboColumn® format is designed for use with robotic workstations for HTPD work. Praesto RoboColumns® are packed by Atoll GmbH and delivered ready for use. They are 100% quality checked for HETP and asymmetry. Individual laser labelling ensures traceability to corresponding resin and column performance data. The columns are made of biocompatible polypropylene. Praesto SP90 is an agarose-based cation exchange resin. Praesto SP90 is designed for large scale biomolecule purification, including proteins, peptides and oligonucleotides. The relatively large particle size (90 µm), makes Praesto SP90 ideal for capture or intermediate purification of proteins when large sample volumes needs to be processed within a limited time frame.

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Principal Applications

  • Protein purification
  • MAb Purification

Advantages

  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • One Row of 8 prepacked 200µl columns

Typical Physical & Chemical Characteristics:

Polymer Structure Agarose - Strong Cation Exchanger
Appearance Spherical beads
Functional Group Sulfopropyl
Inner diameter 5 mm
Packed bed height 10 mm
Column volume 200 µl
Connector for use with liquid robotic handling stations
Column material Polypropylene
Bed support Filter
Chemical stability All commonly used aqueous buffers, pH 1-14, organic solvents, 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Avoid Halogenated organic solvents, hexane, oxidising agents, anionic detergents
Working pressure (max.) 8 bar
Ionic capacity - medium 0.11 - 0.16 mmol H⁺/ml
Ionic capacity - resin 0.11 - 0.16 mmol/ml
Particle Size - µm 90 µm
Dynamic Binding Capacity (min.) - 6 minutes residence time 50 lgG/ml
Dynamic Binding Capacity (min.)- 3 minutes residence time 50 hlgG/ml
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 800 cm/h
pH stability, CIP (short term) 3 - 14
pH stability, working range 4 - 13
Recommended Storage 4 - 30 °C
Recommended Storage 20% ethanol
Recommended Storage 0.2M sodium acetate